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- ItemAssessing the Antimicrobial Properties of Methanol Extracts of Selected Nigerian Medicinal Plants using Tetrazolium Microplate Assay(African Journal of Pharmaceutical Research and Development, 2025-07-20) Latifat Olabimpe Sidiq, Nkumah Abraham Oluwalana, Rasheed Bolaji Ibrahim, Abdulfatai Temitope Ajiboye, Christiana Oreoluwa Oke and Omonike Oluyemisi OgboleMedicinal plants have made significant contributions to the treatment of infectious diseases. An upsurge in antimicrobial resistance has caused an upsurge in to the demand for the identification and development of novel antimicrobial agents. This study aimed to investigate the antimicrobial activity of methanol leaf extracts of viz; Deterium microcarpum Guill. & Perr, Lophira alata Banks ex Gaertn, Hibiscus tiliaceus L. and Ricinus communis L. The Minimal Inhibitory Concentration (MIC) values for the test microorganisms, Staphylococcus aureus (ATCC6571), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC 27853), and Bacillus subtilis (ATCC3366), and isolates of Trichophyton rubrum and Candida albican were determined using the modified rapid p-iodonitrotetrazolium chloride (INT) colorimetric assay. The antimicrobial activity of the extracts varied against all the test microorganisms with MIC values ranging between 125.00-7.81μg/mL. Detarium microcarpum extract exhibited complete growth inhibition at the lowest extract concentration with a MIC value of 7.81 μg/mL on C. albican while Hibiscus tiliaceus and Ricinus communis had the highest growth inhibition on T. rubrum at the MIC value of 15.63 μg/mL. After subculturing, all the extracts displayed Minimum Fungicidal Concentration (MFC) ranged from 31.25-62.50 μg/mL with T. rubrum. Hibiscus tiliaceus, Detarium microcarpum and Lophira alata exhibited strong Minimum Bactericidal Concentration (MBC) on E. coli with an MBC value of 62.50 μg/mL. This study justifies the usage of these plants in ethnomedicine to treat a variety of infectious disorders, based on the broad-spectrum antimicrobial activity these plants have demonstrated.
- ItemEffects of Zinc Oxide Nanoparticles on Chlorophyll Content, Growth Attributes, Antioxidant Enzyme Activities And Bioaccumulation of Common Bean (Phaseolus Vulgaris L.) Grown In Soi...(IOSR Journal of Environmental Science, Toxicology and Food Technology, 2019-10-27) Mariam, A. Akanbi-Gadaa, Clement. O. Ogunkunle, Abiola. O. Ilesanmi, Abiola, G. Femi-Adepoju, “Latifat, O. Sidiq”, Paul. O. FatobaAbstract: The use of conservative products to improve growth and productivity of plants is as old as man. Zinc oxide nanoparticles (ZnONPs) use smart/controlled release and targeted delivery system to improve alot of parameters in plants including yield, thereby making them suitable as fertilizers. This study therefore assessed the effects of ZnONPs on the physiology, chlorophyll content, biochemical activities, antioxidant enzyme activities and bioaccumulation potential of Phaseolus vulgaris after 90 days of exposure to ZnONPs following standard methodologies. The growth parameters examined in this research revealed that there was significant increase at all evaluation times at P <0.05 in all ZnONPs treated plants. Furthermore, there was increase in yield especially at low concentration of 300 mg/kg for all the parameters examined. Flowers and fruits took longer to emerge at high doses of 600 and 1000mg/kg confirming the phytotoxicity tendencies of ZnONPs at higher concentration. Exposure of Phaseolus vulgaris to ZnONPs treatment increased productivity, promoted uptake and bioaccumulation of Zn in the leaf and roots tissues of Phaseolus vulgaris. Futhermore, ZnONPs enhanced the activities of antioxidant enzymes such as superoxide dismutase (SOD), ascorbate peroxidise (APX) and catalase (CAT).
- ItemIn vitro Antioxidant, Total Phenolic Content and Cytotoxicity study of Selected Herbal Medicinal Products in Ilorin Metropolis(Archieve of Basic and Applied Medicine, 2025-07-25) Sidiq L.O., Nkumah A.O. Quadri M.T. and Ogbole O.O.There are no doubts herbal medicines are endowed with bioactive constituents considered to have many therapeutic effects. The usage of herbal medicines increased sporadically as a result of the belief that herbal medicines have a higher safety margin than conventional medicines. In other to improve acceptance of herbal remedies in the treatment of diseases, the study aimed to assess the antioxidant capability, total phenolic content and cytotoxicity of five (5) herbal medicinal products that were sourced from the city of Ilorin. The products were labelled A, B, C, D and E in this study. The Folin-Ciocalteu method was used to determine the total phenolic content, Brine Shrimp Lethality Assay (BSLA) was used to determine the toxicity of the medicinal products, and the 1, 1-diphenyl, 2-picrylhydrazyl (DPPH) radical scavenging assay was applied to evaluate the antioxidant activity of the selected herbal medicinal products. Products C, D and E had higher DPPH radical scavenging activities (IC50 = 54.51± 0.22, 42.30± 0.11 and 39.17± 0.24 μg/mL respectively), which was lower in products A and B (129.53± 0.21 and 180.06± 0.34 μg/mL) compared with Ascorbic acid (7.32± 0.12 μg/mL). Total phenolic content was high in products D and E (315.86±0.22 and 205.13±0.31 mg GAE/g extract. Brine shrimp lethality assay (BSLA) revealed products A, C, D, E were found to be non-toxic to Artemia salina larvae, with LC50 > 1000 μg/mL, except extract of product B which was perceived to be moderately toxic with LC50 = 418.80 ± 0.24 μg/mL. The selected herbal medicinal products possessed reasonable safety, antioxidant capacity and phenolic content.
- ItemPhytochemicals, proximate and elemental analysis, antioxidant and cytotoxic potentials of purple (Capitata F. rubra) and green (Capitata Linn.) Brassica oleraceae (cabbage)(Journal of Pharmacy and Bioresources, 2024-05-25) Thomas ABU, Blessing Obianuju EZEA, Saka Alabi YUSUF, Olorunsola Olasunkanmi BAMIDELE, Latifat Olabimpe SIDIQ, Aminat Omope YUSUF, Omonike Oluyemisi OGBOLEMalnutrition remains a significant impediment to growth in many countries. Exploring the medicinal potentials of exotic vegetables represents a strategic approach to achieving sustainable development goals. This study focused on the evaluation of proximate, elemental, and phytochemical composition, including the antioxidant and cytotoxic potentials of green and purple cabbage varieties. The findings revealed different percentages of moisture, crude fat, fibre, protein, and carbohydrates in both varieties. Calcium, magnesium, potassium, sodium, iron, copper, zinc, saponins, terpenoids, flavonoids, phenolics, tannins, anthraquinones, and steroids were also detected and quantified. The green (IC50=186.3 μg/mL) and purple (IC50=187.6 μg/mL) cabbage extracts demonstrated broad-spectrum 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, as compared with the standard, ascorbic acid (IC50=267 μg/mL). Similarly, the green (IC50=148.7 μg/mL) and purple (IC50=103.9 μg/mL) cabbage extracts exhibited nitric oxide inhibitory activity, as compared with the standard, ascorbic acid (IC50=135.2 μg/mL). The green (CC50=10.6 μg/mL) and purple (CC50=16.66 μg/mL) cabbage extracts exhibited an inhibition on the growth of Rhabdomyosarcoma cell lines as compared with the standard, Vincristine (CC50=0.30 μg/mL). These results support the utilization of the two cabbage varieties as dietary supplements, potentially aiding in the discovery of anticancer drugs and the management of other disease conditions owing to their antioxidant properties.
- ItemAntibacterial Activities of Methanol and Ethanol Extracts of Moringa oleifera(Lam) Leaves(Federal University of Kashere, Gombe State, 2024) Akanbi-Gada, M. A.; Abubakar, F.A.; Ajiboye A.T; Olorukooba H.O; Jimoh F.A; Olabamiji S.T; Yahaya Z.O; Adenekan A; Amubieya, O.FMoringa oleifera is known for its therapeutic potential, particularly in treating bacterial infections. This study aimed to determine the Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) of Moringa oleifera extracts (ethanol and methanol) on two bacterial strains: Escherichia coli (gram-negative) and Staphylococcus aureus (gram-positive). The extracts were tested at concentrations of 200, 150, 100, 50, and 25 mg/ml. Phytochemical analysis was also conducted. Results showed that Moringa extracts exhibited significant antibacterial activity, with the methanol extract showing an MIC and MBC of 200 mg/ml, and the ethanol extract showing an MIC and MBC of 150 mg/ml. The zones of inhibition were 19 mm (E. coli) and 18 mm (S. aureus) for methanol extract, and 18 mm (E. coli) and 17 mm (S. aureus) for ethanol extract. These results were similar to those of Ciprofloxacin (a standard antibiotic), indicating that Moringaextracts have strong antimicrobial properties. The study suggests that Moringa oleifera could be used as an alternative or adjunct to conventional antibiotics. Future studies should explore the synergistic effects of combining Moringa extracts with existing antibiotics to enhance their effectiveness and combat antibiotic resistance