Browsing by Author "Olugbemi Tope Olaniyan"

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    Adansonia digitata aqueous leaf extract ameliorates dexamethasone-induced testicular injury in male Wistar rats
    (Asian Pacific Journal of Reproduction, 2021) Olugbemi Tope Olaniyan
    Objective: To evaluate the effects of aqueous leaf extract of Adansonia (A.) digitata L on dexamethasone-induced testicular damage in male Wistar rats. Methods: Twenty adult male Wistar rats weighing 170-190 g were divided into four groups. Group栺received 0.5 mL of phosphate buffer orally for 28 days and served as the normal control group; group栻 received 10 mg/kg of dexamethasone (a synthetic glucocorticoid) intraperitoneally for 7 days and 0.5 mL of phosphate buffer orally for 21 days, group栿 received 10 mg/kg of dexamethasone for 7 days and 800 mg/kg of A. digitata extract orally for 21 days; group 桇 received 10 mg/kg of dexamethasone for 7 days and 300 mg/kg of vitamin-E orally for 21 days. Dexamethasone was administered intra-peritoneally for 7 days and all administration lasted for 28 days. The rats were sacrificed by anesthesia with diethyl ether and the testes of each animal were harvested. The testis was homogenized in 0.25 M sucrose at 4°曟 for biochemical and histological analyses. Results: Administration of dexamethasone significantly decreased body weight, glucose-6-phosphate dehydrogenase (G6PDH), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (P<0.05), and significantly increased malondialdehyde (MDA) activities (P<0.05). The degeneration in the population of spermatogonia and vacuolation and abnormal widening of the interstitial spaces were observed in the rats treated with dexamethasone. However, administration of A. digitata significantly increased SOD, GPx, G6PDH, and LDH levels, significantly decreased MDA activities and improved the histoarchitecture of the testis (P<0.05). Conclusions: A. digitata may have an ameliorative effect on dexamethasone-induced testicular damage in Wistar rats because of its anti-inflammatory and antioxidant properties.
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    Aqueous extract of Adansonia digitata prevents Cadmium Chloride-induced testicular damage in Wistar Rats
    (J Basic Clin Physiol Pharmacology - de gruyter, 2021) Olugbemi Tope Olaniyan
    Objectives: The role of aqueous extract of Adansonia dig itata was investigated against cadmium chloride-induced testicular damage in Wistar Rats. Methods: Thirty (30) male Wistar Rats weighing (150–170) were divided into six groups (n=5). Group A served as con trol and received oral administration of phosphate buffer saline; groupBreceived800mg/kgA.digitataonly;groupC wereinjectedintraperitoneallywithsingledose2mg/kgb.w cadmium chloride; group D were injected intraperitoneally with single dose2 mg/kgb.wcadmiumchlorideandtreated with 800 mg/kg aqueous extract of A. digitata; groupE received 300 mg/kg vitamin E only; group F were injected intraperitoneally with single dose 2 mg/kg b.w cadmium chloride and treated with 300 mg/kg vitamin E. After 21 days, the animals were sacrificed by cervical dislocation, thetestes wereexcisedfixedinBouinsfluidsforhistological analysis and the other homogenized in5%sucrose solution for determination of tissue malondialdehyde (MDA) and antioxidant enzyme activity, biochemical assay. Results: The group treated with cadmium chloride plus A. digitata caused significant decrease in MDA levels with significant increase (p<0.05) in antioxidant activities and biochemical enzymes when compared to cadmium chlo ride only group. Conclusions: Aqueous extract of A. digitata appears to have ameliorative effect against cadmium chloride induced testicular damage. This could be attributed to the presence of polyphenolic compound
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    Impact of COVID-19 on assisted reproductive technologies and its multifacet influence on global bioeconomy
    (Journal of Reproductive Healthcare and Medicine, 2021) Olugbemi Tope Olaniyan
    Several nations of the world have issued instructions such as travel restrictions, border closure, and lockdown, plus other directives proposing that non-essential care must be withdrawn including assisted reproductive services, in an attempt to identify resources to ascertain the dissemination of SARS-CoV-2. This has led to massive shortage in medical supplies, inappropriate service delivery, hike in price, decrease in staff work load, salary cut, decrease in the utilization of qualitative maternal, and reproductive health-care services thereby creating high risk on reproductive health and global bioeconomy. The search for right candidate for the management of coronavirus disease 2019 and several reproductive health challenges begins with the screening of natural products to identify novel active constituent. Moreover, there is need to pay more attention to crucial phytochemical, bioactive fractions, phytoanalysis, and phytopharmacological investigation for effective drug discovery most especially these bioresources from beneficial microorganisms, plants, and ocean deposits that could help in mitigation of SARS-CoV-2 and reproduction health challenges through chemoinformatics, informatics, synthetic biology, nanotechnology, and metabolomics hence boosting the global economy.
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    Protective effects of methanol extract of Plukenetia conophora seeds and 4H-Pyran-4-One 2,3-Dihydro-3,5-Dihydroxy-6-Methyl on the reproductive function of male Wistar rats treated with cadmium chloride
    (JBRA Assisted Reproduction, 2018) Olugbemi Tope Olaniyan
    Objectives: Male infertility caused by exposure to heavy metals is a current global issue. Exposure to cad mium chloride (CdCl2 ) negatively affects the male repro ductive system. Many infertile people, especially in devel oping countries, resort to folkloric treatment. Plukenetia conophora is used in Nigerian folk medicine to promote fertility. This study investigated the effects of Plukenetia conophora (PC) and 4H-Pyran-4-One 2,3-Dihydro-3,5-Di hydroxy-6-Methyl (DDMP) on Wistar rats with cadmium chloride-induced testicular damage. Methods: Forty-two male Wistar rats (150-190g) were divided into seven groups (n=6) and treated daily for 54 days as follows: Controls (normal saline); CdCl2 (2mg/kg single IP dose); CdCl2 + 200 mg/kg vitamin E; CdCl2 + 100 or 200 mg/kg PC; and CdCl2 + 25 or 50 mg/kg DDMP. The rats were sacrificed 55 days after the start of the study; Samples were collected for analysis. Biochemical parame ters malondialdehyde, nitric oxide, antioxidant enzymes, and proton pumps were measured by spectrophotometry. Reproductive hormones were measured using ELISA. Data were analysed using ANOVA and differences in mean val ues were considered significant at p<0.05. Results: Significant increases in sperm count, motility, and viability were observed in the groups given CdCl2 +Vi tamin E, CdCl2 +PC or CdCl2 +DDMP as compared with the CdCl2 group. Malondialdehyde and nitric oxide levels in the groups treated with CdCl2 +PC or CdCl2 +DDMP decreased significantly when compared with the group given CdCl2 . Significant increases were observed in antioxidant en zymes, proton pump, and testosterone in the groups treat ed with CdCl2 +PC or CdCl2 +DDMP, respectively. Conclusion: Plukenetia conophora alleviated male re productive toxicity induced by cadmium chloride in Wistar rats. 4H-Pyran-4-One 2,3-Dihydro-3,5-Dihydroxy-6-Meth yl present in Plukenetia conophora may be responsible for the ameliorative effects.