Protective effects of methanol extract of Plukenetia conophora seeds and 4H-Pyran-4-One 2,3-Dihydro-3,5-Dihydroxy-6-Methyl on the reproductive function of male Wistar rats treated with cadmium chloride
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Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
JBRA Assisted Reproduction
Abstract
Objectives: Male infertility caused by exposure to
heavy metals is a current global issue. Exposure to cad
mium chloride (CdCl2
) negatively affects the male repro
ductive system. Many infertile people, especially in devel
oping countries, resort to folkloric treatment. Plukenetia
conophora is used in Nigerian folk medicine to promote
fertility. This study investigated the effects of Plukenetia
conophora (PC) and 4H-Pyran-4-One 2,3-Dihydro-3,5-Di
hydroxy-6-Methyl (DDMP) on Wistar rats with cadmium
chloride-induced testicular damage.
Methods: Forty-two male Wistar rats (150-190g) were
divided into seven groups (n=6) and treated daily for 54
days as follows: Controls (normal saline); CdCl2
(2mg/kg
single IP dose); CdCl2
+ 200 mg/kg vitamin E; CdCl2
+ 100
or 200 mg/kg PC; and CdCl2
+ 25 or 50 mg/kg DDMP. The
rats were sacrificed 55 days after the start of the study;
Samples were collected for analysis. Biochemical parame
ters malondialdehyde, nitric oxide, antioxidant enzymes,
and proton pumps were measured by spectrophotometry.
Reproductive hormones were measured using ELISA. Data
were analysed using ANOVA and differences in mean val
ues were considered significant at p<0.05.
Results: Significant increases in sperm count, motility,
and viability were observed in the groups given CdCl2
+Vi
tamin E, CdCl2
+PC or CdCl2
+DDMP as compared with the
CdCl2
group. Malondialdehyde and nitric oxide levels in the
groups treated with CdCl2
+PC or CdCl2
+DDMP decreased
significantly when compared with the group given CdCl2
.
Significant increases were observed in antioxidant en
zymes, proton pump, and testosterone in the groups treat
ed with CdCl2
+PC or CdCl2
+DDMP, respectively.
Conclusion: Plukenetia conophora alleviated male re
productive toxicity induced by cadmium chloride in Wistar
rats. 4H-Pyran-4-One 2,3-Dihydro-3,5-Dihydroxy-6-Meth
yl present in Plukenetia conophora may be responsible for
the ameliorative effects.
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NIL
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30