Adansonia digitata aqueous leaf extract ameliorates dexamethasone-induced testicular injury in male Wistar rats
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Date
2021
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Asian Pacific Journal of Reproduction
Abstract
Objective: To evaluate the effects of aqueous leaf extract of
Adansonia (A.) digitata L on dexamethasone-induced testicular
damage in male Wistar rats.
Methods: Twenty adult male Wistar rats weighing 170-190 g
were divided into four groups. Group栺received 0.5 mL of
phosphate buffer orally for 28 days and served as the normal
control group; group栻 received 10 mg/kg of dexamethasone (a
synthetic glucocorticoid) intraperitoneally for 7 days and 0.5 mL of
phosphate buffer orally for 21 days, group栿 received 10 mg/kg
of dexamethasone for 7 days and 800 mg/kg of A. digitata extract
orally for 21 days; group 桇 received 10 mg/kg of dexamethasone
for 7 days and 300 mg/kg of vitamin-E orally for 21 days.
Dexamethasone was administered intra-peritoneally for 7 days and
all administration lasted for 28 days. The rats were sacrificed by
anesthesia with diethyl ether and the testes of each animal were
harvested. The testis was homogenized in 0.25 M sucrose at 4°曟 for
biochemical and histological analyses.
Results: Administration of dexamethasone significantly decreased
body weight, glucose-6-phosphate dehydrogenase (G6PDH),
lactate dehydrogenase (LDH), superoxide dismutase (SOD),
and glutathione peroxidase (GPx) (P<0.05), and significantly
increased malondialdehyde (MDA) activities (P<0.05). The
degeneration in the population of spermatogonia and vacuolation
and abnormal widening of the interstitial spaces were observed in
the rats treated with dexamethasone. However, administration of
A. digitata significantly increased SOD, GPx, G6PDH, and LDH
levels, significantly decreased MDA activities and improved the
histoarchitecture of the testis (P<0.05).
Conclusions: A. digitata may have an ameliorative effect on
dexamethasone-induced testicular damage in Wistar rats because of
its anti-inflammatory and antioxidant properties.
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