Vitamin E modulates androgen receptor gene expression to attenuate ovarian dysfunctions in a rat model of dehydroepiandrosterone-induced polycystic ovary
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Date
2023
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Asian Pacific Journal of Reproduction - Wolters Kluwer- Medknow.
Abstract
Objective: To investigate the protective effect of vitamin E in
dehydroepiandrosterone (DHEA)-induced polycystic ovary in
rats.
Methods: Premature female Wistar rats were randomly allocated
into four groups, with 7 rats in each group. Group栺 received corn
oil (vehicle) and served as the control group; group栻 received
0.2 mL of 0.06 mg/g DHEA in corn oil; group栿 received
200 mg/kg vitamin E; group 桇 received DHEA plus vitamin
E. All treatments lasted for 15 days, with DHEA administered
subcutaneously, while vitamin E and corn oil were administered
orally. After the experiment, serum samples and ovaries were
harvested for biochemical, immunohistochemical, hormonal, and
histological analysis. The ovarian mRNA expression of androgen
receptor was analyzed by reverse transcriptase quantitative
polymerase chain reaction (qPCR).
Results: The antioxidant and metabolic enzyme activity
significantly decreased in the DHEA-treated rats compared to
the control rats (P<0.05). Administration of vitamin E to DHEA
treated rats significantly decreased cytokines and malondialdehyde
compared to the DHEA-treated rats. The histological analysis
showed reduced atretic and cystic ovaries, increased E-cadherin and
Bcl-2 expression, and reduced expression of Bax in the DHEA
treated rats co-treated with vitamin E. The mRNA expression
of androgen receptor was upregulated in the DHEA-treated rats
compared to the control rats. Conclusions: Vitamin E ameliorates the hyperandrogenic effect of
DHEA-induced polycystic ovaries via metabolic, antioxidant, and
anti-apoptotic pathways.
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