Histological Changes and Lipid Profile Analysis in Male Rats Exposed High Fat Diet
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Date
2022-11-19
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Basic and Clinical Toxicology
Abstract
Abstract
Background: High Fat Diet (HFD) is associated with development of cardiovascular, metabolic, liver and central nervous system diseases. The study is aimed at evaluating the effects of high fat diets on lipid profile and selected organs in male wistar rats.
Methodology: 24 rats were randomly assigned into 4 groups. Group A received Normal Rat Chow diet + Distilled Water (DW), Group B fed 25% HFD + DW, Group C fed with 50% HFD + DW and Group D fed 100% HFD + DW. Animal’s weights were measured pre and post-treatment. Rats were anaesthetized (diethylether) and sacrificed after 12 hours fast. The blood samples taken from the orbital sinus, centrifuged and serum obtained was used for the assay of lipoprotein A, Total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL). The brain, liver and heart were excised and processed for histology.
Results: Result showed the presence of a significant dose dependent increase (p < 0. 0 5) in the body weight, concentrations of TC, TAG, LDL, lipoprotein A and decrease in the concentrations of HDL in HFD groups compared with the control. Histological results from HFD treated rats have showed no histological abnormalities of the liver, brain and heart tissues.
Conclusion: Intake of high fat diets in this study altered serum lipid profile with significant weight gain - a risk factors for cardiovascular and metabolic diseases.
Keywords: High Fat Diet, Lipid profile, Lipoprotein A, Histological Changes
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Keywords
Citation
Ibrahim, M., Abubakar, L., Kola, O., Titilope, F., Garuba, W., Popoola, A., Adunmo, G., Ibrahim, S., Busari, A., Balogun, M., & Abdulrasak, N. (2022). Histological Changes and Lipid Profile Analysis in Male Rats Exposed High Fat Diet. 1, 25-30.