Bioremediation of Spent Engine Oil on Selected Contaminated Soils within Ilorin Metropolis
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Date
2020
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Abstract
The research aimed to investigate the bioremediation of spent
engine oil on selected contaminated soils within Ilorin metropolis.
To achieve this, soil samples were collected from three (3)
mechanic workshops along Taiwo axis within the metropolis. The
soil samples were then subjected to bioremediation using the landfarming
approach. The physicochemical parameters of the soil
samples before and after bioremediation were analyzed using
standard methods. Bacteria were isolated using standard
procedures and identified using biochemical tests and molecular
methods. Results for the physicochemical parameters of the soil
samples before bioremediation include particle size (all sandy in
nature); pH (6.00 ± 0.14 - 6.20 ± 0.14); Organic carbon (14.65 ±
3.20 - 17.54 ± 1.87), Organic matter (33.50 ± 0.85 - 43.45 ± 9.12)
and heavy metals (ND - 11.74 ± 0.07). Values after bioremediation
for pH, organic carbon, organic matter and heavy metals were 8.25
± 0.07 - 8.90 ± 0.14, 13.07 ± 0.05 - 13.25 ± 0.84, 37.25 ± 1.06 -
44.80 ± 1.13, ND - 9.40 ± 0.04 respectively. Values for bacterial
count before and after bioremediation of the soil samples were
8.00 ± 1.41 - 67.50 ± 2.12 x 105 CFU/mL and 6.50 ± 2.12 - 164.00
± 11.31 x 105 CFU/mL respectively. Bacterial isolates were
identified as Pseudomonas sp., Enterobacter sp., Acinetobacter sp., and
Bacillus sp. while the hydrocarbon-utilizing bacteria were identified
as Thalassospira mesophila strain JCM 18969; Pseudomonas fluorescens
F113; Siccibacter turicensis LMG 23730; Pseudomonas Zeshuii strain
KACC 15471; Pseudomonas stutzeri strain CGMCC 1.1803 and
Marinobacter hydrocarbonoclasticus strain ATCC 49840. In conclusion,
the bacteria isolates effectively bioremediated the spent engine oil
contaminated soils with a reduction of hydrocarbon pollutants.