Scholarly Publication

Permanent URI for this collection

https://kwasuspace.kwasu.edu.ng/handle/123456789/116

Browse

Recent Submissions

Now showing 1 - 5 of 181
  • No Thumbnail Available
    Item
    Basic Techniques in Microbiology: A Laboratory Handbook (Second Edition) Prefa
    (Kwasu press, 2025) Prof. S. Awe; Dr. A.E. Ajiboye; Dr. M.R. Adedayo; Dr. A.T. Ajao; Dr. W.T. Aborisade; Dr. F.A. Jimoh; Dr. Z.B. Abdulsalam; Mrs. S.A. Abdulmumini; Mrs. B.O. Yusuf-Salihu; *Mr. M.M. Abdulrazaq*; Mrs. A.O. Amupitan; Mrs. L. Uthman-Saheed; Mrs D.O. Gabriel; Mrs. H.A. Mohammed; Mrs. G.B. Yusuf; Mr. S.S. Jumah
  • Thumbnail Image
    Item
    Microbiology Laboratory Manual
    (KWASU PRESS, 2024-05-01) Awe S. ; Ajiboye A.E.; Adedayo M.R.; Ajao A.T.; Aborisade W.T.; Jimoh F.A.; Abdulsalam Z.B.; Abdulmumini S.A.; Yusuf-Salihu B.O.; Abdulrazaq M.M.; Amupitan A.O.; Uthman-Saheed L.; Muhammad A. ; Said R.O.; Ahmed A.O.; Gabriel D.O.; Mohammed H.A.; Yusuf G.B.; Jumah S.S.
    Microbiology is a critical field that advances our understanding of health, disease and the environment through the study of microorganisms. This manual serves as a comprehensive guide to essential techniques used in microbiological laboratories, starting with foundational practices and safety protocols that ensure a safe and efficient working environment. Proper handling and maintenance of the microscope, a vital tool in microbiology, are also covered to ensure accurate and effective observation of microorganisms. The manual explores the practical aspects of aseptic techniques to prevent contamination, culture media preparation to support microbial growth and methods for isolating pure cultures. Detailed instructions on various staining techniques enhance the visibility and contrast of microorganisms, while enumeration methods provide accurate quantification essential for many microbiological studies. Each section is designed to provide thorough guidance on specific laboratory practices, from sterilization methods to the interpretation of staining results. Additionally, the manual explores the cultural characteristics of bacteria, crucial for their identification and classification based on observable traits such as shape, size, colour and texture. This resource aims to equip students, researchers and professionals with the necessary skills and knowledge to conduct microbiological research effectively, fostering the development of proficient microbiologists capable of advancing our understanding of the microbial world. To facilitate a quick learning platform for students, immediate Exercises following practical classes are included, reinforcing the concepts and techniques learned to foster their expertise in the microbial world.
  • Thumbnail Image
    Item
    Microbiology Laboratory Workshop
    (KWASU PRESS, 2024-05-01) Authors: Prof. S. Awe; Prof. A.E. Ajiboye; Prof. M.R. Adedayo; Dr. A.T. Ajao; Dr. W.T. Aborisade; Dr. F.A. Jimoh; Dr. Z.B. Abdulsalam; Dr. S.A. Abdulmumini; Dr. B.O. Yusuf-Salihu; Mr. M.M. Abdulrazaq; Mrs. A.O. Amupitan; Mrs. L. Uthman-Saheed; Mr.A . Muhammad; Mrs.R.O.Said; Mr.A.O.Ahmed; Mrs D.O. Gabriel; Mrs. H.A. Mohammed; Mrs. G.B. Yusuf; Mr. S.S. Jumah
  • Thumbnail Image
    Item
    Bacteraemia in a Nigerian hospital: Implementing antimicrobial resistance surveillance
    (Journal of Public Health in Africa, 2025-02-07)
    Background: Surveillance of drug-resistant infections is crucial for antimicrobial resistance (AMR) control. Implementing surveillance in low- and middle-income countries (LMICs) is challenging. Aim: To investigate bacteraemia and describe AMR surveillance. Setting: Tertiary healthcare facility. Methods: Case finding was by WHO Global AMR and Use Surveillance System (GLASS). Blood samples were processed between May 2017 and June 2018, using BACTEC blood culture system. Bacterial identification, antibiotic susceptibility testing and detection of AMR genes followed standard protocols. Results: Aerobic blood cultures were conducted in a third of clinical sepsis cases (n = 601/1851), of which 114 (19.0%) were true positives, with a 2.2% contamination rate. Pathogens recovered included six priority blood pathogens reportable to WHO GLASS. Sixteen (30.2%) of 53 Gram-negative isolates were extended-spectrum beta-lactamase producers, predominantly harbouring blaCTX-M, three (5.7%) were AmpC beta-lactamase producers, and 20 (37.7%) were carbapenem-resistant, predominantly harbouring blaKPC . Twenty-nine (50.9%) of 57 Staphylococcus aureus isolates were methicillin-resistant; 17 (58.6%) of these harboured mecA genes. Hospital-acquired infection (odds ratio [OR] = 0.3, 95% confidence interval [CI]=0.1–0.7, p = 0.004) was identified as a predisposing factor for the development of multidrug-resistant (MDR) bacteraemia. Bacteraemia with MDR organisms was significantly associated with mortality (OR = 3.8, 95% CI = 1.6–9.1, p = 0.001). Conclusion: A wide variety of bacteria are responsible for bacteraemia in our setting, with more than half being multidrug-resistant. Bacteraemia with multidrug-resistant organisms was significantly associated with mortality, hence, the need for this AMR surveillance initiative. Contribution: Implementing healthcare facility-based surveillance of AMR in LMICs is achievable despite limited microbiological laboratory capacity. Keywords: bacteraemia; antimicrobial-resistance; surveillance; healthcare-facility; Nigeria; GLASS; multidrug resistance; implementation.
  • Thumbnail Image
    Item
    Antimicrobial Effects of Ocimum gratissimum Extracts on the Spoilage Organisms Isolated from Yoghurt Samples
    (UJMR, 2025-06-14) Amupitan Adefunke Olawumi; AjiboyeAdeyinka Elizabeth; Adedayo Majekodunmi Rachael; Amupitan Adewale Alex
    Ocimum gratissimum (OG) is recognised for its nutritional, antioxidant, and antimicrobial properties, making it a strong candidate for natural food preservation. This study evaluated the effects of ethanolic and aqueous OG leaf extracts against spoilage organisms isolated from laboratory-prepared yoghurt samples stored at room temperature for ten days. The agar well diffusion technique was employed for antimicrobial screening, while the broth microdilution method was used to determine both minimum inhibitory concentration (MIC) and minimum cidal concentration (MCC). The isolated organisms included Bacillus subtilis, Bacillus spp., Saccharomyces cerevisiae, Saccharomyces bulderi, Pichia kudriavzevii, Aspergillus niger, and Aspergillus flavus, identified by standard microbiological methods. The aqueous extract at a concentration as low as 62.50 mg/mL inhibited Bacillus subtilis, Saccharomyces bulderi, Pichia kudriavzevii, and Saccharomyces cerevisiae, with inhibition zones ranging from 5.00±0.27 to 6.20±0.87 mm. The ethanolic extract exhibited antimicrobial effects against Bacillus subtilis, Bacillus spp., Saccharomyces bulderi, Pichia kudriavzevii, and Saccharomyces cerevisiae at the same concentration (62.50 mg/mL), but with larger inhibition zones ranging from 5.30±0.45 to 11.35±2.10 mm. The aqueous extract showed substantial inhibition of Bacillus subtilis, Saccharomyces bulderi, and Pichia kudriavzevii, with an MIC of 62.50 mg/mL. In contrast, the ethanolic extract demonstrated an MIC of 31. 25 mg/mL for Bacillus subtilis, Bacillus spp., and Aspergillus niger. The MCC of the aqueous extract was 250 mg/mL for Bacillus subtilis, Saccharomyces bulderi, Pichia kudriavzevii, Aspergillus flavus, and Saccharomyces cerevisiae, while the MCC of the ethanolic extract was 125 mg/mL for Bacillus subtilis and Bacillus spp. Overall, the OG extracts exhibited substantial antimicrobial effects, effectively inhibiting the growth of spoilage organisms isolated from stored yoghurt samples. Harnessing the potential of OG can lead to an extended shelf life for yoghurt, positioning OG as a promising natural preservative