ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF Adansonia digitata STEM BARK EXTRACT ON SOME CLINICAL ISOLATES
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Date
2020
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Obafemi Awolowo University, Ile Ife
Abstract
Adansonia digitata is a massive and deciduous tree with a height of about 24 m and of significant economic
importance. The antimicrobial and phytochemical screening of the aqueous and ethyl-acetate extract of stem
bark of the plant were determined on some clinical isolates. The stem bark of the plant was collected and washed
o
properly before drying at 28 C. The pulverised stem bark was extracted with water and ethyl-acetate and
screened for phytochemicals (qualitative and quantitative) using standard methods. The clinical isolates used
were identified as Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli and Candida albicans. The antimicrobial
activities of the crude extracts were carried out using the agar well diffusion methods. The minimum inhibitory
concentration (MIC), minimum bactericidal and fungicidal concentrations were carried out using standard
methods. The aqueous extract exhibited a higher zone of inhibition against S. aureus (14.00 ± 0.57 mm) at a
concentration of 200 mg/ml while a zone of inhibition of 11.66±0.33 mm was observed for E. coli using ethyl acetate extract. Candida albicans had a zone of inhibition of 11.66±0.88 mmand 11.00±0.57 mm using aqueous
and ethyl-acetate extracts respectively at 200 mg/ml. The MIC was 200 mg/ml for the crude extracts against the
clinical isolates. The qualitative ethyl-acetate phytochemical screening revealed the presence of phenol,
flavonoids, tannins, alkaloids, saponin and terpenoids. Phenol had the highest concentration of 2.02±0.25
mg/ml while terpenoids had a value of 1.38±0.02 mg/ml. Aqueous and ethyl-acetate extract of A. digitata
possess significant antimicrobial activity against E. coli, S. aureus and C. albicans. However, K. pneumoniae showed
resistance to ethyl acetate extract
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Ajiboye et al. 2020: Antimicrobial Activity and Phytochemical Screening of Adansonia digitata Stem Bark