Department Of Zoology

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    Acaricidal Efficacy of Cassia sieberiana DC (Caesalpinaceae) Stem Bark Aqueous Extract on Hyalomma KOCH, 1844 (Acari: Ixodidae) Reproductive Stages
    (Arid Zone Journal of Basic and Applied Research, 2025-02) Abdullahi A. Biu; Maimunatu A. Abdulkadir; Saidu I.Ngulde; ThankGod E. Onyiche; Umar A. Maina; Raliat Aliyu; Aliyu M. Danzaria
    One of the most significant challenges facing tick control through chemical acaricides is resistance and environmental pollution, encouraging the need for natural plant products that are less toxic and environment friendly. This study was conducted to determine the acaricidal efficacy of Cassia sieberiana stem bark aqueous extract on Hyalomma species using immersion testing for egg-hatch inhibition and adult mortality. Both tests used ascending extract concentrations of 5%, 10%, 15% and 20%, at durations of 1, 2, 3, 4, 5, 6, 7 and 8 hours. Egg-hatch inhibition peaked at 5% concentration at 8hrs, 10% and 15% at 7 and 8hrs and 20% at 5, 6, 7 and 8hrs while adult mortality was highest at 20% concentration. There was a positive correlation between graded extract concentration, immersion period, egg-hatch inhibition and adult mortality. The LC50 values for adult mortality and egg hatch inhibition were 13.1 mg/mL and 13.7 mg/mL and coefficient of determination were y = 3.7x +1.5 and y = 3.062 + 8.12 respectively. Both LC50 values were almost similar indicating a strong association between extract concentration and acaricidal activity in vitro.
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    Impact of climate change on the spatial distribution of endemic legume species of the Guineo-Congolian forest, Africa
    (2021-03) Oyebanji, O. O., Salako, G., Nneji, L. M., Oladipo, S. O., Bolarinwa, K. A., Chukwuma, E. C., Ayoola, A. O., Olagunju, T. E., Ighodalo, D. J., & Nnej
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    DNA barcoding and species delimitation of butterflies (Lepidoptera) from Nigeria.
    (2020-12) Nneji, L. M., Adeola, A. C., Ayoola, A. O., Oladipo, S.O., Wang, Y.-Y., Malann, Y. D., Anyaele, O., Nneji, I. C., Rahman, M. M., & Olory, C. S
    Accurate identification of species is a prerequisite for successful biodiversity management and further genetic studies. Species identification techniques often require both morphological diagnostics and molecular tools, such as DNA barcoding, for correct identification. In particular, the use of the subunit I of the mitochondrial cytochrome c oxidase (COI) gene for DNA barcoding has proven useful in species identification for insects. However, to date, no studies have been carried out on the DNA barcoding of Nigerian butterflies. We evaluated the utility of DNA barcoding applied for the first time to 735 butterfly specimens from southern Nigeria. In total, 699 DNA barcodes, resulting in a record of 116 species belonging to 57 genera, were generated. Our study sample comprised 807 DNA barcodes based on sequences generated from our current study and 108 others retrieved from BOLD. Different molecular analyses, including genetic distance-based evaluation (Neighbor-Joining, Maximum Likelihood and Bayesian trees) and species delimitation tests (TaxonDNA, Automated Barcode Gap Discovery, General Mixed Yule-Coalescent, and Bayesian Poisson Tree Processes) were performed to accurately identify and delineate species. The genetic distance-based analyses resulted in 163 well-separated clusters consisting of 147 described and 16 unidentified species. Our findings indicate that about 90.20% of the butterfly species were explicitly discriminated using DNA barcodes. Also, our field collections reported the first country records of ten butterfly species—Acraea serena, Amauris cf. dannfelti, Aterica galena extensa, Axione tjoane rubescens, Charaxes galleyanus, Papilio lormieri lormeri, Pentila alba, Precis actia, Precis tugela, and Tagiades flesus. Further, DNA barcodes revealed a high mitochondrial intraspecific divergence of more than 3% in Bicyclus vulgaris vulgaris and Colotis evagore. Furthermore, our result revealed an overall high haplotype (gene) diversity (0.9764), suggesting that DNA barcoding can provide information at a population level for Nigerian butterflies. The present study confirms the efficiency of DNA barcoding for identifying butterflies from Nigeria. To gain a better understanding of regional variation in DNA barcodes of this biogeographically complex area, future work should expand the DNA barcode reference library to include all butterfly species from Nigeria as well as surrounding countries. Also, further studies, involving relevant genetic and eco-morphological datasets, are required to understand processes governing mitochondrial intraspecific divergences reported in some species complexes.
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