OPTIMISATION OF N-ETHYL-N-NITROSOUREA (ENU) DOSE FOR MUTAGENESIS IN THE NIGERIAN YORUBA ECOTYPE CHICKEN
| dc.contributor.author | Adesina, O. M. | |
| dc.contributor.author | Ikeobi, C. O. N. | |
| dc.contributor.author | Oduntan, R. O. | |
| dc.contributor.author | Hanotte, O. | |
| dc.contributor.author | Toye, A. A. | |
| dc.date.accessioned | 2025-03-12T12:44:47Z | |
| dc.date.available | 2025-03-12T12:44:47Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | The otimal dose and regime of ENU for mutagenesis in the chicken was determined through dose titration (0mg/kg body weight, control group; 100mg; 200mg; 300mg and 400mg/kg body weight) and regime variation (single shot, half dose in each of two cosecutive weeks, and 1/3 dose in each of 3 consecutive weeks) studies in one hundred and twenty (120) 25 week-old improved Nigerian local chicken cocks. The study was laid out as a 5 x 3 factorial layout of treatments within a completely randomised experimetal design. Eight individually tagged cocks were assigned to each treatment. Mortality was recorded over 48 hours from the point of each ENU administration. From week 5 – 14 following completion of the assigned ENU dose/regime, semen was collected from each ENU mutagenized cock twice weekly and used to inseminate two Issa brown commercial layers hen i.e. 1 : 2 mating ratio and a total of 240 hens used. Eggs were collected over a period of 5 days in each week and set in an egg incubator to test fertility (candling at day 12). The data showed that ENU dose and regime independently and significantly (p < 0.05) control fertility. The 400mg dose produced significantly lower fertility than the control group throughout the examined period, indicating an absence of recovery of fertility to levels comparable to the Control group. There was transient loss of fertility in the 300mg dose group relative to the control group in weeks 6, 8, 10, 11 and 13 and at week 14, fertility was not significantly (p > 0.05) different from the control group. The 200mg dose showed no significant loss of fertility throughout the experimental period, and this was mirrored in the 100mg group except at week 10. ENU regime significantly (p < 0.05) detrmined fertility at weeks 7 and 9 – 11 only. Administration of 1/3 dose in each of 3 consecutive weeks resulted in the longest period of consistent loss of fertility (weeks 9 – 11) relative to the group given the assigned dose in a single shot regime. Cummulative mortality within 48 hours after ENU administration was significantly determined by dose (0mg, 100mg, 200mg < 300mg < 400mg) and regime (1 dose > 2 doses, 3 doses). The 300 mg dose administered in 3 equal fractions over 3 weeks resulted in transient sterility and recovery and is therefore recommended for ENU mutagenesis in Nigerian Local chicken. | |
| dc.identifier.isbn | 978-978-958-959-3 | |
| dc.identifier.uri | https://kwasuspace.kwasu.edu.ng/handle/123456789/4611 | |
| dc.language.iso | en | |
| dc.publisher | Proceedings of 42nd annual Conference of Nigerian Society for Animal Production: In Emerging Challenges facing Animal Agriculture in Nigeria and the way forward. (Eds. A. A. Adeloye, E. O. oyawoye, A. A. Toye, B. T. Adesina). | |
| dc.title | OPTIMISATION OF N-ETHYL-N-NITROSOUREA (ENU) DOSE FOR MUTAGENESIS IN THE NIGERIAN YORUBA ECOTYPE CHICKEN | |
| dc.type | Article |