Dietary antioxidants impact DDT resistance in Drosophila melanogaster
| dc.contributor.author | Ojo James Adebayo | |
| dc.date.accessioned | 2023-07-20T16:35:53Z | |
| dc.date.available | 2023-07-20T16:35:53Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Insects experience a diversity of subtoxic and/or toxic xenobiotics through exposure to pesticides and, in the case of herbivorous insects, through plant defensive compounds in their diets. Many insects are also concurrently exposed to antioxidants in their diets. The impact of dietary antioxidants on the toxicity of xenobiotics in insects is not well understood, in part due to the challenge of developing appropriate systems in which doses and exposure times (of both the antioxidants and the xenobiotics) can be controlled and outcomes can be easily measured. However, in Drosophila melanogaster, a well-established insect model system, both dietary factors and pesticide exposure can be easily controlled. Additionally, the mode of action and xenobiotic metabolism of dichlorodiphenyltrichloroethane (DDT), a highly persistent neurotoxic organochlorine insecticide that is detected widely in the environment, have been well studied in DDT-susceptible and -resistant strains. Using a glass-vial bioassay system with blue diet as the food source, seven compounds with known antioxidant effects (ascorbic acid, β-carotene, glutathione, α-lipoic acid, melatonin, minocycline, and serotonin) were orally tested for their impact on DDT toxicity across three strains of D. melanogaster: one highly susceptible to DDT (Canton-S), one mildly susceptible (91-C), and one highly resistant (91-R). Three of the antioxidants (serotonin, ascorbic acid, and β-carotene) significantly impacted the toxicity of DDT in one or more strains. Fly strain and gender, antioxidant type, and antioxidant dose all affected the relative toxicity of DDT. Our work demonstrates that dietary antioxidants can potentially alter the toxicity of a xenobiotic in an insect population. | |
| dc.description.sponsorship | This work was primarily supported by MSU Foundation Professor and AgBioResearch funds provided to BRP and partially funded by an Egyptian government grant (No: SAB-2154). The funder provided support in the form of salaries for authors [GA, WS, BRP], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section | |
| dc.identifier.citation | Abdu-Allah GAM, Seong KM, Mittapalli O, Ojo JA, Sun W, Posos-Parra O, et al. (2020) Dietary antioxidants impact DDT resistance in Drosophila melanogaster. PLoS ONE 15(8): e0237986. https:// doi.org/10.1371/journal.pone.0237986 | |
| dc.identifier.uri | https://kwasuspace.kwasu.edu.ng/handle/123456789/670 | |
| dc.publisher | PloS ONE | |
| dc.title | Dietary antioxidants impact DDT resistance in Drosophila melanogaster |