ANTIBACTERIAL POTENTIAL OF TAPINANTHUS BANGWENSIS (AFRICA MISTLETOE) ETHANOLIC EXTRACTS ON SELECTED CLINICAL ISOLATES
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Date
2024-07-01
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Publisher
Nature and Science
Abstract
Antibiotics resistance has been recognized as an emerging problem worldwide both in human and veterinary medicine. Tapinanthus bangwensis is a well-known evergreen parasitic plant and an excellent medicinal plant belonging to the family Loranthanceae used as a remedy for several human and animal ailments that include stomach ache, diarrhea, dysentery, wound, arthritis, epilepsy and cancer traditionally. This study aimed was to assess the antibacterial potential of T. bangwensis on pathogenic bacteria and also study the qualitative and quantitative phytochemicals inherent in the plant. Ethanolic extracts of T. bangwensis leaf and stem was challenged with clinical isolates of Staphylococcus aureus, Escherichia coli and Klebsiella Pneumoniae. Antibacterial activity was investigated using agar well diffusion method while qualitative and quantitative phytochemical screening were carried out using Gas and mass spectroscopy method. Antibacterial effect of T. bangwensis leaf and stem showed various inhibitory effect against the microbial isolates. The leaf extract exhibited a higher zones of inhibition against Escherichia coli (18.70 ± 0.6mm) at a concentration of 1000 mg/ml while a zone of inhibition of 16.30±0.6mm was observed on E. coli using stem extract. Klebsiella Pneumoniae and Staphylococcus aureus had a zone of inhibition of 15.70±0.5 mm using stem extract respectively at 1000 mg/ml. The MIC was 250mg/ml for the crude extracts against the clinical isolates. The Minimum Bactericidal Concentration (MBC) recorded for the leaf extract was observed to be 1000mg/ml for Staphylococcus aureus and 500mg/ml for Escherichia coli and Klebsiella Pneumoniae whereas MBC for the stem extract recorded 500mg/ml for Staphylococcus aureus and 1000mg/ml for Escherichia coli and Klebsiella Pneumoniae. The result obtained from the phytochemical screening revealed the presence of tannin, steroids, phenolics, flavonoids, anthocyanin, coumarin, terpenoids, glycoside, and alkaloid among others. Steroids and flavonoids was predominant in the leaf with the value of 309.68 ±0.94 mg/kg and 274.92 ± 1.09 mg/kg respectively while Glycoside had the least (12.67±0.01 mg/kg). In the stem 109.02±1.89 mg/kg recorded as the highest value with the lowest of 0.22±0.01 mg/kg for Saponin. These results could suggest the promising chemopreventive use of T. bangwensis and some of its active principles in the treatment of infections.
Description
ANTIBACTERIAL POTENTIAL OF TAPINANTHUS BANGWENSIS (AFRICA MISTLETOE) ETHANOLIC EXTRACTS ON SELECTED CLINICAL ISOLATES WAS ASSESSED