ORAL SUPPLEMENTATION OF CISSUS POLPUNEAAND CITRUS AURANTIFOLIA ENHANCED SERUM BIOCHEMISTRY AND OXIDATIVE STATUS OF TROPICAL RABBITS
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Date
2025
Journal Title
Journal ISSN
Volume Title
Publisher
Association of Organic Agriculture Practitioners of Nigeria (NOAN)
Abstract
The effect of Cissus populnea stem bark extract (CpSTe), with or without the addition of Citrus
aurantifolia extract (CAe), was evaluated on the serum biochemistry and oxidative status of rabbits in
the tropics over 34 days. Twenty-four (24) New Zealand White female rabbits were divided into four
groups of six rabbits each, designated as Treatment 1-4 (T1-T4). T1 received no extract (Control), T2
was given 2 mL of CAe per kg body weight, T3 received 2 mL of CpSTe per kg body weight, and T4
was given a combination of CAe (1 mL) + CpSTe (1 mL)/kg body weight. At the end of the
experiment, a 5 mL blood sample was collected from the marginal ear vein of three rabbits per
treatment using a syringe and needle; out of which 3 mLwas dropped into plain bottles to obtain serum
for biochemical analysis, while a 2 mL blood sample was dropped into heparinized bottles for
hematological assessments The proximate and phytochemical analyses of CPST revealed
carbohydrate (67.85%) and tannin (126.60 mg/100 g) as the predominant constituents. Administration
of CA significantly (p<0.05) increased the total serum cholesterol in T2, while a decrease was
observed in rabbits under T1 (no administration) and T4. Administering 2 mLof CP (T3) significantly
(p<0.05) raised the levels of superoxide dismutase (SOD), catalase (CAT), and GSH in the rabbits.
The use of CpSTe and CAe separately, rather than combined, could effectively improve the redox
status in tropical rabbits.