Characterization and Partial Purification of Pectinase Produced by Aspergillus niger Using Banana Peel as Carbon Source
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Date
2021
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Abstract
Pectinases are group of enzymes which causes the degradation of pectin. Pectinases are of
environmental and industrial importance due to their biotechnological and commercial potentials in waste
water treatment, brewing industries, textile industries, food industries and paper industries among others.
The present study was aimed at characterization and partial purification of pectinase produced by
Aspergillus niger using banana peel as carbon source under submerged fermentation (SmF) system. Fungi
were isolated from fruit wastes dump sites using standard microbiological technique. The isolates were
characterized morphologically and microscopically before subjecting them to screening for pectinolytic
ability using standard methods. Molecular identifications were carried out on the isolate with the best
pectinolytic ability. Fermentation parameters optimized to increase pectinase activity include; incubation
period, salt supplements, additional carbon source, inoculum sizes, pH, moisture contents and temperature
using standard methods. The isolate with the best pectinolytic ability was identified as Aspergillus niger
strain AH1 with GenBank accession number MK811422. The crude pectinase obtained were partially
purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography methods.
Maximum pectinase activity of 575.37±0.67 U/mg/min/ml was obtained at day 3 of incubation, with
addition of salt supplements, in the presence of sucrose as additional carbon source, 2 ml of 1 × 106 spores
ml-1 of Aspergillus niger, pH 6, 100 ml moisture contents, and temperature of 50 0C. The specific activity
increased with 1.38 fold while recovery yield was 8.94 %. The study confirmed that the isolated
Aspergillus niger strain AH1 can produced maximum pectinase at optimized fermentation parameters and
could be explored for pectinase production