Sustainable fungal production of pectinase in orange peel-based medium: Taguchi optimization, juice clarification and green synthesis of selenium nanoparticles for biomedical applications
| dc.contributor.author | L.S. Beukes | |
| dc.contributor.author | S.A. Abdulmumini | |
| dc.contributor.author | A. Lateef | |
| dc.contributor.author | E.B. Gueguim-Kana | |
| dc.contributor.author | N. Matyumza | |
| dc.date.accessioned | 2025-10-29T11:29:52Z | |
| dc.date.available | 2025-10-29T11:29:52Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Pectinase is a commercially important enzyme that is extensively utilized in the food, textile, and paper in dustries; yet, its large-scale production poses a hurdle due to high cost of pectin for its production. This research utilized Taguchi optimization to improve pectinase production from a non-aflatoxigenic local strain of Aspergillus f lavus, using orange peel as an economical substrate. The improved pectinase was studied for juice clarification and eco-friendly synthesis of selenium nanoparticles (AFP-SeNPs), showcasing its enhanced biotechnological capabilities. The Taguchi L9 orthogonal optimization of pH, inoculum size, substrate concentration, and incu bation time yielded a 397.7 % enhancement in pectinase production with maximum enzyme activity of 921.3 U/ ml, which clarified orange juice by 76.6 %. Herein, we report the first study to synthesize SeNPs using pectinase which were spherical, crystalline, having sizes of 50.97–98.43 nm and absorbed maximally at 268 nm. The nanoparticles inhibited growth of multidrug-resistant bacterial pathogens (Klebsiella oxytoca, Enterobacter cloacae, and Salmonella enterica) up to 25.3 mm and total suppression of fungal growth (Aspergillus flavus, Aspergillus niger, and Penicillium sp.), while displaying 35.9–59.3 % DPPH radical scavenging activities. Addi tionally, AFP-SeNPs successfully prevented blood clot formation in vitro. This research which uniquely combines pectinase-assisted green production of SeNPs has broadened the applications of pectinase. The multifunctional bioactivities of AFP-SeNPs—antimicrobial, antioxidant, and anticoagulant—underscore their significance in biomedicine, pharmaceuticals, and industrial biotechnology. These findings would enhance sustainable enzyme manufacturing and environmentally friendly nanotechnology, providing a cost-efficient and scalable approach for future developments. | |
| dc.identifier.doi | 10.1016/j.microb.2025.100400 | |
| dc.identifier.issn | 2950-1946 | |
| dc.identifier.uri | https://kwasuspace.kwasu.edu.ng/handle/123456789/6226 | |
| dc.publisher | Elsievier | |
| dc.relation.ispartof | The Microbe | |
| dc.title | Sustainable fungal production of pectinase in orange peel-based medium: Taguchi optimization, juice clarification and green synthesis of selenium nanoparticles for biomedical applications | |
| dc.type | journal-article | |
| oaire.citation.volume | 7 |