Isolation, Identification and Screening of Humic Acid Producing Fungi from Soil Environment of Oil palm (Elaeis guineensis) Associated with Empty Fruit Bunches
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Date
2023-12
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Department of Microbiology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Kastina.
Abstract
The demand for sustainable and sustainable biotechnological processes has developed as a result
of a growing interest in converting agricultural waste into valuable products. In this study, fungal
strains were isolated from soil environment of oil palm empty fruit bunches and screened for
humic acid production. In Osun state, Nigeria, soil sample was taken from a local palm oil mill.
Different colonies of fungi were obtained for further research using the serial dilution plating
procedure. Plate screening was used to evaluate the fungal biomass as well as colony diameter of
the fungal isolates, and a submerged fermentation test was used to measure the amount of humic
acid concentration the isolates produced. Fungal isolates were identified by using molecular
methods and morphological analysis. The fungi species Aspergillus niger, Rhizopus stolonifer, and
Penicillium chrysogenum were isolated from the soil environment. When compared to Rhizopus
stolonifer, Aspergillus niger grew more superiorly in terms of zone diameter (26.5 1.50 mm) and
biomass (2.23 0.16 g), while Penicillium chrysogenum showed no growth. Throughout the
fermentation process, Aspergillus niger also produced a greater quantity of humic acid, with
concentrations varying from 0.70 mg/l to 2.20 mg/l. Aspergillus niger strain HR18's existence was
confirmed by molecular analysis. This experiment proved that Aspergillus niger can efficiently
produce humic acid from OPEFB, providing a useful method for waste utilization and sustainable
practices.
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Citation
Kawata, Hassan Musa , Omojasola, P. F., Ajiboye A.E ., Adedayo, M.R. and Bale, Sheriffdeen Issa (2023)