Prevalence and antibiotic resistance of Escherichia coli O157:H7 in beef at a commercial slaughterhouse in Moro, Kwara State, Nigeria

Simple item page
dc.contributor.authorAjuwon. B.I.
dc.contributor.authorBabatunde, S.K.
dc.contributor.authorKolawole, O.M.
dc.contributor.authorAjiboye, A.E.
dc.contributor.authorLawal, A.H
dc.date.accessioned2025-01-28T21:14:10Z
dc.date.available2025-01-28T21:14:10Z
dc.date.issued2021
dc.description.abstractBackground. Gastroenteritis due to foodborne disease is a leading cause of death in developing countries. In Nigeria, there is an increasing demand for beef. Yet, there is no surveillance for Escherichia coli O157:H7 contamination of raw beef and little is known about the carriage of this pathogen in Nigeria’s livestock. Methods. A total of 415 samples, including 180 cow carcass swabs, 180 caecal content samples, 16 water samples, 25 hand swabs and 14 knife swabs were collected at a large abattoir in the Moro region of Kwara State, Nigeria. The samples were enriched in modified tryptone broth containing novobiocine, and plated onto Sorbitol–MacConkey agar (Oxoid SR0172E) supple- mented with 0.05 mg l−1 cefixime and 2.5 mg l−1 potassium tellurite (Oxoid) (CT-SMAC). Indole- producing isolates were confirmed serologically by serotyping with antisera specific for the O157 and H7 antigens. The E. coli O157:H7 isolates were further tested for their susceptibility to antibiotic agents using the disc diffusion method. Commercially available Gram-negative multi- discs (Oxoid) comprising nitrofurantoin (30 μg), ampicillin (5 μg), ceftazidime (30 μg), gentamicin (10 μg), ciprofloxacin (5 μg), augmen- tin (30 μg), ofloxacin (5 μg) and cefuroxime (30 μg) were tested. Results. Overall, 16 (3.9 %) samples were contaminated with E. coli O157:H7, of which 10 (5.6 %) were isolated from carcass swabs, 4 (2.2 %) from caecal content samples and 2 (12.5 %) from water. All isolates were multidrug-resistant (MDR), with resist- ance to ampicillin, ceftazidime and cefuroxime being the most common. Conclusion. This study provides evidence to suggest that E. coli O157:H7 exists in the beef production chain. The pathogen reveals a high frequency of multidrug resistance, suggesting that consumers and handlers of such meat are at risk of contract- ing antibiotic- resistant E. coli O157:H7-associated foodborne disease. Routine monitoring of antibiotic resistance is critical to uncovering novel therapeutic strategies that will help inform clinical practice guidelines.
dc.identifier.citationAjuwon. B.I., Babatunde, S.K., Kolawole, O.M. Ajiboye, A.E. and Lawal, A.H. (2021). Prevalence and antibiotic resistance of Escherichia coli O157:H7 in beef at a commercial slaughterhouse in Moro, Kwara State, Nigeria. Access Microbiology 3:000289
dc.identifier.urihttps://kwasuspace.kwasu.edu.ng/handle/123456789/3502
dc.language.isoen
dc.publisherMicrobiology Society
dc.titlePrevalence and antibiotic resistance of Escherichia coli O157:H7 in beef at a commercial slaughterhouse in Moro, Kwara State, Nigeria
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Prevalence and antibiotic resistance in Beef Ajuwon.pdf
Size:
220.25 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
Scholarly Publication