Purification and Partial Characterization of Cellulase Produced by Aspergillus niger Cultured on Vitellaria paradoxa shells

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dc.contributor.authorSulyman A. O, Iyanda A. Y, Opasola O. A, Adedayo O, Aladodo, R. A, Balogun A, 1Ajibola O. A, Olukotun O. Z and Hammed B. A
dc.date.accessioned2025-02-04T14:33:47Z
dc.date.available2025-02-04T14:33:47Z
dc.date.issued2017-06-20
dc.descriptionCellulase (EC 3.2.1.4), an enzyme responsible for the hydrolysis of cellulose (the major component of plant framework) and related cello-oligosaccharide derivatives, can be produced from a wide range of agricultural wastes. Cellulase has a wide variety of industrial applications such as in textile (Gusakov et al., 2000; Belghith et al., 2001), detergents (Adrio and Demain, 2014; Shen et al., 2017), pulp and paper (Lee et al., 2017), and animal feed industries (Yuangklang et al., 2017).
dc.description.abstractThis research investigated the purification and partial characterization of cellulase produced by Aspergillus niger cultured on Vitellaria paradoxa shells. Cellulase (endoglucanase) from A. niger was produced under optimum fermentation conditions at 35 °C, pH 4.7, V. paradoxa, 4 g/L, inoculum size of 10 mm and the fermentation media incubated for 120 hours. The crude endoglucanase obtained were partially purified by subjecting to ammonium sulphate precipitation, dialysis and gel filtration chromatography for further purification. The effect of temperature and pH on the activity of purified endoglucanase was determined. Cellulase was purified to 734.33 folds by Sephadex G-100 column chromatography with a specific activity and yield of 4.406 U/mg and 63.03% respectively. Fractions 4 and 7 contained the highest endoglucanase activity out of 18 fractions collected and the two fractions were pooled for further analysis. The activity of purified endoglucanase was optimum at a temperature of 40 °C and pH 5. Therefore, the purified endoglucanase produced may be explored in detergent industry.
dc.description.sponsorshipSELF
dc.identifier.citationSulyman A.O, Iyanda A.Y, Opasola O.A, Adedayo O,Aladodo R.A, Balogun A, Ajibola O.A, Olukotun O.Z and Hammed B.A (2017). Purification and partial Characterization of cellulose produced by Aspergillus niger cultured on Vitellaria paradoxa shells. Fountain Journal of Natural and Applied Sciences 6(2): 24-30.
dc.identifier.issnISSN:2354-337X(Online),2350-1863(Print)
dc.identifier.urihttps://kwasuspace.kwasu.edu.ng/handle/123456789/3897
dc.language.isoen_US
dc.publisherA publication of College of Natural and Applied Sciences, Fountain University, Osogbo, Nigeria.
dc.relation.ispartofseries6(2); 24-30
dc.titlePurification and Partial Characterization of Cellulase Produced by Aspergillus niger Cultured on Vitellaria paradoxa shells
dc.typeArticle
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