Purification and Partial Characterization of Cellulase Produced by Aspergillus niger Cultured on Vitellaria paradoxa shells
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Date
2017-06-20
Journal Title
Journal ISSN
Volume Title
Publisher
A publication of College of Natural and Applied Sciences, Fountain University, Osogbo, Nigeria.
Abstract
This research investigated the purification and partial characterization of cellulase produced by Aspergillus
niger cultured on Vitellaria paradoxa shells. Cellulase (endoglucanase) from A. niger was produced under
optimum fermentation conditions at 35 °C, pH 4.7, V. paradoxa, 4 g/L, inoculum size of 10 mm and the
fermentation media incubated for 120 hours. The crude endoglucanase obtained were partially purified by
subjecting to ammonium sulphate precipitation, dialysis and gel filtration chromatography for further
purification. The effect of temperature and pH on the activity of purified endoglucanase was determined.
Cellulase was purified to 734.33 folds by Sephadex G-100 column chromatography with a specific activity
and yield of 4.406 U/mg and 63.03% respectively. Fractions 4 and 7 contained the highest endoglucanase
activity out of 18 fractions collected and the two fractions were pooled for further analysis. The activity of
purified endoglucanase was optimum at a temperature of 40 °C and pH 5. Therefore, the purified
endoglucanase produced may be explored in detergent industry.
Description
Cellulase (EC 3.2.1.4), an enzyme responsible for the
hydrolysis of cellulose (the major component of plant
framework) and related cello-oligosaccharide
derivatives, can be produced from a wide range of
agricultural wastes. Cellulase has a wide variety of
industrial applications such as in textile (Gusakov et
al., 2000; Belghith et al., 2001), detergents (Adrio
and Demain, 2014; Shen et al., 2017), pulp and paper
(Lee et al., 2017), and animal feed industries
(Yuangklang et al., 2017).
Keywords
Citation
Sulyman A.O, Iyanda A.Y, Opasola O.A, Adedayo O,Aladodo R.A, Balogun A, Ajibola O.A, Olukotun O.Z and Hammed B.A (2017). Purification and partial Characterization of cellulose produced by Aspergillus niger cultured on Vitellaria paradoxa shells. Fountain Journal of Natural and Applied Sciences 6(2): 24-30.