Solid State Fermentation of Plantain Peels for Bioethanol Production by Saccharomyces cerevisiae and Aspergillus niger
Loading...
Date
2024-12
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Federal University, Oye- Ekiti
Abstract
Plantain peels and other agricultural wastes are in abundance and are a nuisance to the
environment. This study evaluated the possibility of ethanol production from plantain
peels in solid-state fermentation using naturally occurring fungi and Saccharomyces
cerevisiae. Naturally occurring fungi were obtained from the plantain peels by solid-state
fermentation and were identified with macroscopic and microscopic methods. Proximate
composition of the plantain peel was analyzed using standard methods. Co-cultures of
isolated fungus and S. cerevisiae were adopted for ethanol production in solid-state
fermentation for an incubation period of five days. Assay was done daily using the
fermented filtrate, which was used to determine the reducing sugar, ethanol yield, total
titratable acid, pH, and specific gravity using standard microbiological procedures. The
plantain peel has carbohydrate content of 41.66%, protein, 6.98%, and moisture, 7.08%.
The highest reducing sugar, 31.20 ± 0.14 mg/L, was obtained on day 1 by co-cultures of
S. cerevisiae and A. niger. The highest ethanol yield, 7.22 ± 0.13 mg/L, was obtained on
day 5 by co-cultures of S. cerevisiae and A. niger. Total titratable acid (TTA) ranged
between 33.64 ± 0.06 and 59.04 ± 0.11 mg/L. The pH ranged between 3.91 ± 0.01 and
5.18 ± 0.01. The specific gravity ranged between 0.925 ± 0.06 kg/m3
and 0.995 ± 0.01
kg/m3
. In conclusion, co-cultures of S. cerevisiae and A. niger have great potential for
bioethanol production using plantain peels in solid-state fermentation.
Description
Keywords
Citation
Ajiboye et al. (2024)