Effect of pH and Temperature on the Activity of Lipase Produced by Aspergillus niger Cultured on Citrus sinensis Peel
| dc.contributor.author | Abdulhakeem O. Sulyman, Yusuf A. Iyanda, Abdulazeez Balogun, Jamui B. Ahmad, Raliat A. Aladodo, Rukayat Aliyu and Ramat O. Ajadi | |
| dc.date.accessioned | 2025-02-02T21:04:51Z | |
| dc.date.available | 2025-02-02T21:04:51Z | |
| dc.date.issued | 2018-02-26 | |
| dc.description.abstract | This study investigated effect of pH and temperature on the activity of lipase produced by Aspergillus niger during fermentation of Citrus sinensis peel. To obtain crude enzyme solutions, 100 ml of enriched media were mixed with 5 g of Citrus sinensis peel in 250 ml Erlenmeyer flask. Then, this mix was inoculated with 10 cfu/ml of microorganism and incubated at 35°C for 168 hours. After the seventh day of incubation, the crude enzyme was collected by filtration followed by centrifugation. The crude enzyme solutions obtained were subjected to ammonium sulphate precipitation, dialysis and gel filtration chromatography for further purification. The Michalis-Menten kinetic constants Km and Vmax for purified lipase were determined by varying concentrations of p-nitrophenyl laurate (p-NPL). Evaluation of effect of temperature and pH on purified enzyme activity was also carry out. This purification procedure resulted in 11.81 fold purification of lipase with 14.10% final yield. Sixteen (16) fractions of 5 ml each were collected after gel chromatography, however, only fraction 8 gave higher lipase activity. The values of Km and Vmax as calculated from the Lineweaver-Burke plot using p-nitrophenyl laurate (p-NPL) as substrate were 1.229 mg/ml and 8.097U/ml respectively. Maximum lipase activity of a purified lipase was observed in a wide range of temperature and pH values with optimum temperature of 39°C and pH 9. | |
| dc.identifier.issn | 2319-3077 | |
| dc.identifier.uri | https://kwasuspace.kwasu.edu.ng/handle/123456789/3857 | |
| dc.language.iso | en | |
| dc.title | Effect of pH and Temperature on the Activity of Lipase Produced by Aspergillus niger Cultured on Citrus sinensis Peel | |
| dc.type | Article |