Antibacterial Efficacy of Aqueous and Ethanolic Extracts of Hibiscus sabdariffa and Ocimum gratissimum on some selected Gram-Negative Bacteria

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dc.contributor.authorAgbabiaka, T. O., Sule, I.O., Uthman, L. and Odebisi-Omokanye, M.B
dc.date.accessioned2025-01-24T21:54:13Z
dc.date.available2025-01-24T21:54:13Z
dc.date.issued2020
dc.description.abstractThis study is aimed at looking at the most effective between extracts of Hibiscus sabdariffa calyces and Ocimum gratissimum leaves on Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Proteus vulgaris and Klebsiella pneumoniae. Antibacterial efficacy of distilled water and ethanolic extracts of Hibiscus sabdariffa calyces and Ocimum gratissimum leaves on the above-named organisms were evaluated by agar well diffusion technique and broth dilution method. The antibacterial efficacy of these extracts was compared with the commercially available antibiotics. The dried residue obtained was reconstituted with distilled water to a concentration of 400mg/ml. Some of the organisms showed varying degree of resistances to the antibiotics while some were sensitive. The aqueous and ethanolic extracts of H. sabdariffa and the ethanolic extract of O. gratissimum inhibited growth of the organisms at concentrations of 100 -400 mg/ml (zones of inhibition ranged between 6 and 24 mm). The minimal inhibitory concentrations (MICs) of the extracts were between 24 and 93.75 mg/ml. This revealed that ethanolic extract of O. gratissimum, the aqueous and ethanolic extracts of H. sabdariffa have antimicrobial activity on all the organisms and probably could be used to control infections associated with them.
dc.identifier.urihttps://kwasuspace.kwasu.edu.ng/handle/123456789/3112
dc.publisherCentrepoint Journal (Science Edition)
dc.titleAntibacterial Efficacy of Aqueous and Ethanolic Extracts of Hibiscus sabdariffa and Ocimum gratissimum on some selected Gram-Negative Bacteria
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