Biosynthesis of Ag and TiO2 nanoparticles and the evaluation of their antibacterial activities
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Date
2022-04-20
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Elsevier
Abstract
In this study, Pseudomonas aeruginosa was used for the synthesis of silver and titanium oxide nanoparticles (Ag
and TiO2 NPs). The nanoparticles were prepared by mixing the supernatant solution of the cultured organism and
the solution of silver nitrate and titanium tetrabutoxide. UV–Vis spectroscopic analysis gave absorption peak at
wavelength of 414 nm and 390 nm for Ag and TiO2 NPs respectively. The FTIR spectra gave absorption bands
attributed to N–H stretch of the peptide linkages, O–H stretch of carboxylic acid, amide bend, and –C––O
stretch which may have emanated from the micro-organism. The powdered X-ray diffraction (PXRD) studies
revealed the crystalline nature of AgNPs and TiO2 NPs while microscopic studies showed spherical and dispersed
morphologies with average particle size of 7.27 nm and 6.83 nm for Ag and TiO2 NPs respectively. Antibacterial
analysis of the synthesized nanoparticles showed broad spectrum inhibition against disease causing microor ganisms with TiO2NPs exhibiting excellent results more than AgNPs and the control drugs
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