Lipidomic profile of testicular and epididymal reserves oF West African Dwarf goats

Abstract
This study presents a comparative lipidomic analysis of the testicular and epididymal reserves of the West African Dwarf (WAD) goat using gas chromatography–mass spectrometry (GC–MS). Lipid extracts from paired testicular and epididymal tail tissues were analyzed to determine the composition and distribution of fatty acids. Twenty two lipid compounds were identified across all samples, including saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), and sterols. The major fatty acids detected were palmitic acid (C16:0), stearic acid (C18:0), linoleic acid (C18:2), and oleic acid (C18:1). The relative abundance of PUFAs was higher in the epididymal tail compared to the testes, indicating their potential role in sperm membrane fluidity and motility. Cholesterol, an essential membrane component, was relatively consistent across tissues. These findings contribute to understanding lipid metabolism in male reproductive physiology of WAD goats and provide a biochemical basis for fertility enhancement, breed conservation, and improved semen cryopreservation strategies.
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