Antimicrobial activity and phytochemical screening of the fruit pulp of Dialium guineense (Velvet Tamarind) on some microbial isolates
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Date
2015-08
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Publisher
Journal of Microbiology and Antimicrobials
Abstract
The antimicrobial activities of the aqueous, ethanol and n-hexane fruit pulp extracts of Dialium
guineense were evaluated against clinical isolates of Klebsiella pneumonia, Staphylococus aureus,
Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Candida albicans using the agar well
diffusion technique. The results reveal that the crude aqueous and ethanol extracts possess
antimicrobial activities on the tested organisms with the exception of n-hexane extract which showed no
zone of inhibition. The highest zone of inhibition diameter at 24.67 mm of the ethanol extract and 19.33
mm of the aqueous extract was recorded against C. albicans while S. aureus showed the lowest
inhibition zone to the aqueous extract with 7.33 mm in diameter. However, statistical analysis indicates
no significance as P>0.05. The minimum inhibitory concentration (MIC) of the aqueous and ethanol
extracts to the isolates was between 100 - 200 mg/ml with only C. albicans at 50 mg/ml of the ethanol
extract. Also, the minimum lethal concentration (MLC) of the aqueous and ethanol extracts on majority
of the organisms was above 200 mg/ml but P. aeruginosa and P. mirabilis showed MLC at 200 mg/ml
and, C. albicans at 100 mg/ml of the ethanol extract. Meanwhile, only C. albicans showed MLC to the
aqueous extract at 100 mg/ml. In addition, the phytochemical screening revealed the presence of
flavonoids, alkaloids, tannin, saponins, oxalates and glycosides. The results of this work suggest
further exploitation of the fruit pulp of D. guineense to possibly unveil its potential use for the treatment
of diseases.
Description
the study was on Antimicrobial activity and phytochemical screening of the fruit pulp of Dialium guineense (Velvet Tamarind) on some microbial isolates