The Studies of Antioxidant and Antimicrobial Potentials of the Leaf Extract of Bauhinia monandra Plant
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Date
2015-06-17
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Natural Product Chemistry Research
Abstract
In the present study, the dried leaf (144g) of B. monandra plant was extracted with 95% ethanol. The crude extract
was partitioned into ethyl acetate and n-hexane layers to afford fractions with golden brown and greenish yellow
colours respectively. The fractions were concentrated and coded EFBM and HFBM. The preliminary phytochemical
screening conducted on the crude extract and ethyl acetate fraction (EFBM) revealed the presence of lavonoids,
tannins, steroids, terpenoids, saponin, cardiac glycoside and phenols. The antioxidant activity of EFBM and HFBM was
evaluated using DPPH radical scavenging assay with ascorbic acid as a reference standard. However, the result of
analysis revealed that EFBM exhibited high antioxidant (IC50
µg/mL=0.010) activity than HFBM (IC50
µg/m = 5.564) and
reference standard ascorbic acid (IC50
µg/mL=30). The antibacterial activity of EFBM and HFBM against three gram
negative bacterial strains (Eschericha coli, Pseudomonas aeruginosa and Klebsillia oxytoca) was also evaluated and
it was found that EFBM exhibited highest antibacterial potential. In contrast to this, HFBM showed negligible or zero
activity against all the bacteria strains. The results of the GC-MS analyses of EFBM and HFBM showed the presence
of nine different phytochemicals. In EFBM, oleic acid which is classiied as monounsaturated omega-9-fatty acid with
percentage concentration, (40.76%) was the major compound as identiied by the GC-MS and 4-hydroxy-5-methyl
3-propyl-2-hexanone with percentage concentration, (42.70%) was identiied as the major compound in HFBM. The
results of this studies demonstrated that folk medicine can be as effective as modern medicine to combat pathogenic
microorganisms. The millenarian use of the leaf of this plant in folk medicine suggests that it represents an economic
and safe alternative to treat infectious diseases
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Citation
Ajiboye AT, Musa MD, Otun KO, Jimoh AA, Bale AT, et al (2015) The Studies of Antioxidant and Antimicrobial Potentials of the Leaf Extract of Bauhinia monandra Plant. Nat Prod Chem Res 3: 180. doi:10.4172/2329-6836.1000180