Molecular Epidemiology of Mycobacterium tuberculosis among Pulmonary Tuberculosis Patients in Ilorin, Nigeria
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Date
2020-07-22
Journal Title
Journal ISSN
Volume Title
Publisher
Scientific Research Publishing Inc. Health, https://www.scirp.org/journal/health
Abstract
Background: Tuberculosis (TB) is an infectious disease, and it is a public health
concern due to the endemic nature of the disease. Nigeria’s health systems still
rely heavily on outdated tuberculosis diagnostic tests, including sputum smear
microscopy and chest radiography. This necessitated the urgent need to explore
a more reliable, effective method that can rapidly enhance the simultaneous detection of Mycobacterium tuberculosis (MTB) and rifampicin (RIF) resistance.
Aims: We evaluated the prevalence of MTB and rifampicin resistance and the
influence of socio-demographic factors. Methods: A longitudinal, descriptive
study that employs the Xpert MTB/RIF automated molecular method for rapid
detection of rifampicin resistance. Secondary data were collected from the
medical record from March 2017 to December 2018. Primary data were collected by direct clinical observations and analysis with a gene expert machine
from January 2019 to March 2019. Results: Study populations were 1893 subjects pooled as secondary data at the Gene Expert laboratory, with a mean age
of 44.92 years and were categorized based on sex, 1125 (59.4%) males and 768
(40.6%) females respectively. The MTB detected were 117 (15.7%) for males
while 76 (9.9%) were positive for females. 948 (84.3%) were negative for
males and 692 (90.1%) negative for females. Also, the categories for age were
grouped into three but the older age groups (29 - 54) and (55 - 80) were more
vulnerable to MTB having a p = 0.506 which is statistically non-significant.
only 14 (5.5%) have RIF while 239 (94.5%) do not have RIF resistance strains.
Logistic regression analysis of MTB detected showed a significant association
(p < 0.05) for sex and a non-significant association (p > 0.05) for age. The
prevalence rate for RIF Resistance is 5.53%, there is a statistical difference p =
0.001 between RIF Resistance and MTB in Ilorin. Conclusion: The utilization
of validated molecular techniques for MTB strain identification has shown a stronger epidemiological impetus for an improved care management outcome specifically in resource-limited areas.