Synthesis, Characterization and α-Amylase Inhibition Study of Substituted Schiff Base and Its Metal Complexes

Abstract

Mechanochemical synthesis is considered as a green and sustainable approach to chemical reactions since it requires little or no solvent, making the process more environmentally friendly by reducing waste and minimizing solvent related hazards. In this study, substituted Schiff base was synthesized from 2-aminophenol and 2- hydroxy-1-naphthaldeyde via mechanochemical synthesis method. The metal complexes were synthesized by reacting the ligand with metal(II) chlorides in ratio 2:1. The synthesized compounds were characterized by colour, melting point, molar conductivity, FT-IR, and UV-Vis spectroscopy. The ligand and its metal complexes were screened for α-amylase inhibitory activity. FT-IR results showed absorption bands in between the ranges 1628-1632 cm-1 and 1585- 1597 cm-1 indicating the formation of azomethine C=N and aromatic C=C respectively. The metal complexes showed M-O absorption bands in between the range 519-579 cm-1 and M-N band in between the range 461-484 cm-1. The synthesized compounds displayed significant inhibitory activities (IC50: 2.00-8.43 mg/mL) against α- amylase comparable to the reference compound, acarbose (IC50: 9.12 mg/mL). These compounds can serve as viable templates in the formulation of new active antidiabetic drugs