ISOLATION AND MOLECULAR IDENTIFICATION OF BACTERIA ASSOCIATED WITH SOIL SURROUNDING THE ROOT OF CITRUS (Citrus sinensis L.) TREE
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Date
2024-06
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Faculty of Physical and Life Sciences FUDMA
Abstract
Microbiological processes occur in the soil around the roots of land plants. This dynamic area, where plants
and microorganisms interact, is crucial for the health and productivity of these plants. This study was aimed at
isolating and molecularly identifying bacteria associated with the soil surrounding the root of an orange tree
growing in the University of Ilorin, Nigeria to be able to know their roles, whether beneficial or harmful.
Securing the soil is a way of having a food-secured world. Soil samples were collected at four different points
from an orange tree trunk into separately labeled Ziploc bags using a separate sterile hand trowel for each
collection. The labeled ziploc bags were brought to the University of Ilorin's Biology Laboratory for
physicochemical analysis of the soil sample and isolation of bacteria using serial dilution method. Results of
the physicochemical assessment of the bulked sample indicated that it is a sandy-loam soil with pH value of
6.7 (slightly neutral). Four bacterial species, i.e. Bacillus cereus, Lysinibacillusmacroides,
Leucobacterkomogatae, and Alcaligenesfaecalis were isolated and identified molecularly. According to
existing literature, the four bacterial species identified in this study are known for their abilities to enhance
plant development and solubilize phosphorus, which are crucial for improving supply of nutrient.
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Citation
Olahan and Ajadi (2024)