Comparison of complete mitochondrial DNA sequences between old and new world strains of the cowpea aphid, Aphis craccivora (Hemiptera: Aphididae).
Loading...
Files
Date
2017-03
Journal Title
Journal ISSN
Volume Title
Publisher
Agri Gene (Elsevier)
Abstract
Mitochondrial DNA provides useful tools for inferring population genetic structure within a species and phyloge netic relationships between species. The complete mitogenome sequences were assembled from strains of the
cowpea aphids, Aphis craccivora, from the old (15,308 bp) and new world (15,305 bp) using shotgun Illumina
HiSeq 2500 sequence reads. The A. craccivora mitogenomes have 13 protein coding genes (PCGs), 22 transfer
RNAs and 2 ribosomal RNAs in the same order and orientation found among Insecta. The two A. craccivora
mitogenomes do not contain long intergenic repeats as found in other members of the family Aphididae. Com parisons between strains indicated a 99.7% sequence identity, wherein 57 and 11 substitutions were respectively
predicted among reads from new and old world strains. Among these substitutions, 31 and 2 were in PCGs of new
world and old world aphids, respectively, and out of which 28 and 2 were nonsynonymous. A majority of the sub stitutions were in NADH Dehydrogenase subunit 1. Phylogenetic analysis of full mitogenomes sequences predict ed that Aphididae as monophyletic and clustered with Pterocommatinae and Aphidinae at subfamily level.