Browsing by Author "Jimoh, F. A."

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    Antibacterial Activities of Methanol and Ethanol Extracts of Moringa oleifera (Lam) Leaves
    (Jewel Journal of Scientific Research , Federal University of Kashere-Nigeria, 2024) Akanbi-Gada, M. A.,; Amubieya O. F.,; Abubakar, F.A.,; Ajiboye, A. T.; Olorukooba, H. O.,; Jimoh, F. A.; Olabamiji, S. T,; Yahaya, Z. O.; Adenekan, A.
    Moringa oleifera is known for its therapeutic potential, particularly in treating bacterial infections. This study aimed to determine the Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) of Moringa oleifera extracts (ethanol and methanol) on two bacterial strains: Escherichia coli (gram-negative) and Staphylococcus aureus (gram-positive). The extracts were tested at concentrations of 200, 150, 100, 50, and 25 mg/ml. Phytochemical analysis was also conducted. Results showed that Moringa extracts exhibited significant antibacterial activity, with the methanol extract showing an MIC and MBC of 200 mg/ml, and the ethanol extract showing an MIC and MBC of 150 mg/ml. The zones of inhibition were 19 mm (E. coli) and 18 mm (S. aureus) for methanol extract, and 18 mm (E. coli) and 17 mm (S. aureus) for ethanol extract. These results were similar to those of Ciprofloxacin (a standard antibiotic), indicating that Moringa extracts have strong antimicrobial properties. The study suggests that Moringa oleifera could be used as an alternative or adjunct to conventional antibiotics. Future studies should explore the synergistic effects of combining Moringa extracts with existing antibiotics to enhance their effectiveness and combat antibiotic resistance.
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    Biosurfactant Production and Characterization by Bacillus altitudinis Strain LCDB-BP3 Isolated from Pesticide-Contaminated Soil in Kwara State, Nigeria
    (Jewel Journal of Scientific Research (JJSR). Federal University of Kashere-Nigeria, 2024) Abdulsalam Z. B.; Ajao, A. T.; Aborisade, W. T.; Jimoh, F. A.
    Microbial biosurfactants have gained attention as promising alternatives for bioremediation, particularly for pesticide-contaminated soils, due to their biodegradability and non-toxic nature. These biosurfactants also hold potential for various industrial applications, including food, petroleum, and fertilizer industries, and may serve therapeutic roles as adjuvants. However, there remains a need to produce biosurfactants using indigenous bacterial isolates to address environmental pollution sustainably. In this study, biosurfactant was produced using Bacillus altitudinis strain LCDB-BP3, identified as a Lambda Cyhalothrin-Degrading Bacterium (LCDB- BP3) in a previous research. The biosurfactant-producing ability of the isolate was confirmed by oil displacement (14.32 cm), emulsification activity (70%), and beta-hemolytic activity on blood agar. Stability tests revealed the biosurfactant's resilience across a wide range of temperatures (up to 105°C), pH (3–13), and salinity (3–7%), making it suitable for diverse environmental conditions. Thin-layer chromatography (TLC) and Fourier-transform infrared (FTIR) spectroscopy further confirmed the presence of functional groups such as O-H, N-H, ester carbonyl, and C-H, supporting the lipopeptide nature of the biosurfactant. These findings highlight the potential of the biosurfactant produced by Bacillus altitudinis strain LCDB-BP3 for use in the bioremediation of pesticide-contaminated soils and offer a greener alternative to chemical agents in industrial settings.