Browsing by Author "Ibrahim Munirudeen"

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    (2025), Volume 11, Issue 1 Niyi et al. 22 https://ejournals.epublishing.ekt.gr/index.php/HealthResJ RESEARCH ARTICLE EFFECTS OF SALBUTAMOL ADMINISTRATION ON THE MORPHOLOGY AND CYTOAR- CHITECTURE OF THE CEREBELLUM AND HIPPOCAMPUS OF ADULT WISTAR RATS
    (Health and Research Journal, 2025-01) Popoola AbdulGafar Niyi; Ibrahim Munirudeen; Ibiyeye Ruqayyah Yetunde; Faniyi Ayodeji Amos; Balogun Musbau Olusesan; Busari Akeem Olayinka; Garba Wasiu Olanrewaju; Ogunwale Kolawole Ayobami; Lawal Abubakar Zubair; Suleiman Ibrahim Eleha; Adunmo Godwin Olawoyin; Nuhu Abdulrazak
    Abstract Background: Salbutamol is the most preferred and widely used drug for treating bronchial asthma and bronchospasm. Its abuse has however been reported amongst users. Most of the side effects reports on salbutamol are clinical based such as headache, tremor, weakness etc. This study, therefore, investigated the sub-acute effect of oral salbutamol on the general morphology of the cerebellum and hippocampus of adult Wistar rats. Method and Material: Twenty adult Male Wistar rats (125 – 224g) were divided into four groups of five rats each. The control (distilled water); the 20mg/kg salbutamol, the 30mg/kg salbutamol, and the 40mg/kg salbutamol groups. Drugs were administered orally for 21 days. The body weight of each animal was monitored throughout the experiment. On day 22, animals were euthanized, brains excised, fixed in 10% buffered formal-saline, cerebelli and hippocampi were identified and processed with Haematoxylin and Eosin staining techniques. Data were analysed by ANOVA at p≤0.05 level of significance using SPSS and results presented as mean ± SEM Results: Results showed that the animals that received 30mg/kg and 40mg/kg salbutamol had significant weight loss. The cerebellum of the 40mg/kg group showed eroded granule cell layer. Hippocampus also revealed pyknotic cells in the pyramidal cell layers at 30mg/kg and 40mg/kg. Conclusions: This study showed that salbutamol at relatively higher doses caused significant weight loss; degeneration of cerebellum granule cells, which might affect motor coordination; and pyknosis of hippocampal pyramidal cells which may affect learning and memory. Keywords: Salbutamol, weight loss, brain, degeneration.
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    Blood Group, Genotype, Malaria, Blood Pressure and Blood Glucose Screening Among Selected Adults of a Community in Kwara State: Implications to Public Health
    (Asian Hematology Research Journal, 2022-06) A. F. Anyiam; O. C. Arinze-Anyiam; P. O. Omosigho; Ibrahim Munirudeen; E. A. Irondi; Emmanuel Ifeanyi Obeagu; E. Obi
    Abstract Background and Objective: Blood groups and Haemoglobin variants vary widely. The malaria scourge is a major setback in Africa. Due to ageing, adults are prone to non-communicable diseases like hypertension. This research was carried out to determine the ABO, Rhesus blood groups and Haemoglobin variants among young people, and to carry out malaria, blood pressure and blood glucose screening among the elderly in Osi Community, Ekiti Local Government Area, Kwara State. Materials and Methods: An empirical research design was done in Osi Community from June 2021 to August 2021. A total of 84 elderly subjects (46 females and 38 males), and 90 young subjects (51 females and 39 males) were selected by purposive sampling. ABO and Rhesus groups were determined using tiles. Haemoglobin variants were determined using alkaline electrophoresis. Malaria screening was done using rapid diagnostic kits. Blood pressure was measured using arm blood pressure monitor. Blood glucose was measured using glucometer. Results: Elderly males mean age was 66.58±10.97 years compared to 62.07±11.74 years for females (p=0.075). Elderly males blood glucose was 112.71±21.85 mg/dl and 105.09±38.46 mg/dl for females (p=0.281). Systolic blood pressureamong elderly males was 147.89±24.45 mmHg and 150.07±22.88 mmHg for females (p=0.676). Diastolic blood pressure among elderly males was 86.61±15.07 mmHg and 86.41±11.12 mmHg for females (p= 0.947). ABO and Rhesus blood group number and percentages among young people were: blood group A 28 (31.1%), B 18 (20.0%), AB 4 (4.4%), O 40 (44.4%), while Rhesus D positive subjects were 87 (96.7%), and Rhesus D negative subjects were 3 (3.3%). Haemoglobin genotype distribution was: AA 66 (73.3%), AS 24 (26.7%), SS 0 (0.0%). Conclusion: The risk of getting heart-related illnesses increases with age. Knowledge of ABO, Rhesus blood groups and haemoglobin genotype variants is vital in healthcare management, medical diagnosis and genetic counselling.
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    Effect of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Renal Profile of Male Wistar Rats
    (Al-Hikmah Journal of Health Sciences (AJOHS), 2021-12) Busari Akeem Olayinka; Salau Fatimoh Abimbola; Bashir Shefiat; Bolaji Moshood; Balogun Morufu Eyitayo; Ibrahim Munirudeen; Garuba Wasiu Olanrewaju; Popoola Niyi Abdulgafar; Adunmo Godwin O.; Lawal Abubakar Z.; Ogunwale Kolawole Tajudeen; Adedokun Kamoru Ademola; Ogundiran Simeon Makinde; Ahmad Muhammad Bello; Mohammed Idris Yahaya; Akinola Franklin Folasele; Adeosun Oyebola Ganiyu
    Abstract Humans have given the leaves of Ocimum gratissimum (O. gratissimum) a great deal of attention due to their vast medical usefulness, culinary application, and pharmacological effects. As a result, the study evaluated the effect of O. gratissimum on renal profile of male Wistar rats. Ten healthy male Wistar rats were randomized into test and control groups comprising of 5 animals each. The test group received 400mg/Kg O. gratissimum leaf extract whereas the control group was fed with normal rat diet and water. The rats were euthanized through cervical dislocation at the end of the experiment and blood sample was collected through heart puncture for serum assessment renal profile while the kidney was excised for histological examination. The Results showed a non-significant at p>0.05 increase in serum electrolytes and urea while significant at p<0.05 decrease in the mean serum creatinine level was observed in the test group compared to the control group. Also, the histological examination of the kidney tissue revealed a mild lymphocytic infiltration in the test group compared to the control group. Conclusively, the study suggested a dose-dependent toxicological effect of O. gratissimum on renal profile.
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    Effects of Aqueous Extract of Ficus Exasperata (Sandpaper Leaf) on Haematological and Some Inflammatory Markers in Wistar Rats Induced Hypertension
    (Al-Hikmah Journal of Health Sciences (AJOHS), 2023-11) Wasiu Olanrewaju Garuba; Gbadebo Maroof Oyeniyi; Ibrahim Munirudeen; Mustapha Abdulrazaq; Akeem Olayinka Busari; Kolawole Tajudeen Ogunwale; olulope Joseph Ogunniyi; Iqmat Abimbola Abdulsalam
    Abstract Hypertension is a global health concern associated with cardiovascular diseases. Ficus exasperata has gained popularity as an alternative therapy to manage hypertension due to its perceived safety and availability. This study evaluated some haematological and inflammatory markers of Ficus exasperata leaf extract in salt-induced hypertensive Wistar rats. Twenty Male Wistar rats were randomly divided into five groups: control, salt-induced + Nifedipine, salt-induced + Ficus exasperata leaf extract (200 mg/kg), salt-induced + Ficus exasperata leaf extract (400 mg/kg), and Ficus exasperate leaf extract (600 mg/kg). Hematological parameters were analyzed using a Hematological autoanalyzer (SYSMEX 1000), Albumin was analyzed using Bromocresogreen (BCG), and C-reactive protein was analyzed using the ELISA method. Data analysis was performed using SPSS version 25, with statistical significance set at p < 0.05. RBC (Red Blood Cell), PCV (Packed Cell Volume), and Hb (Hemoglobin) significantly increased upon treatment with 200 mg/kg body weight of Ficus exasperata leaf extract + 8% salt compared to control at p<0.05. However, the red cell parameters significantly decreased after administering 400 mg/kg and 600 mg/kg body weight of Ficus exasperata at p < 0.05. Albumin increased after treatment with 200 mg/kg body weight of Ficus exasperata leaf extract + 8% salt and 600 mg/kg body weight of Ficus exasperata leaf extract compared to control at p<0.05 but decreased after administering 400 mg/kg body weight of Ficus exasperata leaf extract + 8% salt compared to control at p < 0.05. There was no significant difference in WBC (White Blood Cell) and CRP (C-reactive protein) among the groups p > 0.05. Conclusively, Ficus exasperata leaf extract, particularly at a dose of 200 mg/kg body weight, holds potential as a natural therapy for mitigating salt-induced hypertension and its associated complications
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    Evaluation of Serum Creatine Kinase and Lactate Dehydrogenase Activities in Hypertensive Patients Attending General Hospital Ilorin, Kwara State, Nigeria
    (2021-12) Busari Akeem Olayinka; Onamadi Temitope Ayobami; Ibrahim Munirudeen; Balogun Morufu Eyitayo; Garba Wasiu Olanrewaju; Ogunwale Kolawole Tajudeen; Adunmo Godwin O.; Ahmad Muhammed Bello; Mohammed Idris Yahaya; Nassar Sulaiman Adebayo; Akinola Franklin Folasele; Popoola Niyi Abdulgafar; Lawal Abubakar Zubair; Oyeniyi Maroof Gbadebo; Eleha Suleiman
    Abstract The main factor contributing to morbidity and mortality is hypertension. Although blood pressure is a complicated feature, studies have shown that the activity of adenosine triphosphate (ATP)-generating enzymes like creatine kinase (CK) and lactate dehydrogenase (LDH) is a major predictor of hypertension. As a result, this study evaluated the activities of CK and LDH activities in hypertensive patients attending the General Hospital Ilorin, Kwara State. One hundred and sixty-one (161) hypertensive patients and thirty-nine (39) normotensive subjects were recruited into a case-control study of 200 participants. Body mass index (BMI) was estimated from each participant's measured weight and height, while the blood pressure was determined from the measured systolic blood pressure (SBP) and diastolic blood pressure (DBP) using conventional mercury sphygmomanometer. A 5 mls sample of blood was taken from each subject, and the extracted serum was analyzed using an enzymatic-kinetic method to quantify the CK and LDH activities. Results showed that hypertensive patients had significantly at p<0.05 higher serum CK and LDH activities in hypertensive compared to apparently healthy normotensives. Thus, this study observed significantly increase CK and LDH activities in hypertensive patients
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    Histological Changes and Lipid Profile Analysis in Male Rats Exposed High Fat Diet
    (International Journal of Basic and Clinical Toxicology, 2022-11-19) Ibrahim Munirudeen; Lawal Abubakar Z; Ogunwale Kola A; Falusi Titilope A; Garuba Wasiu O; Popoola Abdulgafar N; Adunmo Godwin.O; Suleiman Ibrahim E; Busari Akeem O; Balogun Musbau O; Nuhu Abdulrasak
    Abstract Background: High Fat Diet (HFD) is associated with development of cardiovascular, metabolic, liver and central nervous system diseases. The study is aimed at evaluating the effects of high fat diets on lipid profile and selected organs in male wistar rats. Methodology: 24 rats were randomly assigned into 4 groups. Group A received Normal Rat Chow diet + Distilled Water (DW), Group B fed 25% HFD + DW, Group C fed with 50% HFD + DW and Group D fed 100% HFD + DW. Animal’s weights were measured pre and post-treatment. Rats were anaesthetized (diethylether) and sacrificed after 12 hours fast. The blood samples taken from the orbital sinus, centrifuged and serum obtained was used for the assay of lipoprotein A, Total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL). The brain, liver and heart were excised and processed for histology. Results: Result showed the presence of a significant dose dependent increase (p < 0. 0 5) in the body weight, concentrations of TC, TAG, LDL, lipoprotein A and decrease in the concentrations of HDL in HFD groups compared with the control. Histological results from HFD treated rats have showed no histological abnormalities of the liver, brain and heart tissues. Conclusion: Intake of high fat diets in this study altered serum lipid profile with significant weight gain - a risk factors for cardiovascular and metabolic diseases. Keywords: High Fat Diet, Lipid profile, Lipoprotein A, Histological Changes
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    Reproductive Endocrinopathies in Nigerian Males with Zero and Three or More Metabolic Syndrome Components
    (Archives of Basic and Applied Medicine, 2017-10-05) Ibrahim Munirudeen; Charles-Davies M.A; Fabian U.A; Fasanmade A.A; Olaniyi J.A; Oyewole O.E; Owolabi M.O; Adebusuyi J.R; Hassan O; Ajobo B.M; Ebesunun M.O; Adigun K; Akinlade K.S; Okoli S.U; Arinola O.G; Agbedana E.O
    Abstract Childlessness has adverse social, psychological and economic impact on affected couples. The contributions of males to infertility account for 40%. Hypogonadism in males has been associated with metabolic syndrome (MS). Although endocrinopathies have been observed in infertile men, similar data in males with MS in the general population is sparse. This study is therefore aimed at evaluating the endocrinopathies in Nigerian males with zero (ZMS) and MS. Sixty-seven male participants aged 18 - 70 years were purposely enrolled for this study. Fifty-two males had ZMS (control) while 15 males had MS. Demography, sexual history, blood pressure, anthropometry were obtained by standard methods. Fasting plasma glucose, triglyceride and high-density lipoprotein cholesterol were determined by enzymatic methods. Sex and pituitary hormones were determined by ELISA. Data analysed were statistically significant at p < 0.05. The MS group 8 (53.3%) had significantly more males with hypogonadism than the controls 2 (23.1%) (p<0.05). Compensatory hypogonadism and suboptimal hypogonadism are the most common endocrinopathies in controls and MS group. 9 (60%) and 5 (33%) of males in the MS group were overweight and obese respectively while 2 (3.8%) and 6 (11.5%) of controls were underweight and overweight. FSH had a direct relationship with age in eugonadal controls and hypogonadal males with MS while LH had an inverse relationship with age in eugonadal males in the MS group. Endocrinopathies are prevalent in Nigerian men irrespective of their metabolic status or age. Key Words: Endocrinopathies, Metabolic Syndrome, Obesity, Hypogonadism, Nigeria
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    Role of apoptotic protease activating factor-1 in CD4+ depletion during HIV progression
    (Qassim University, 2024-05) Wasiu O. Garuba; Adebayo L. Adedeji; Kamoru A. Adedokun; Olubunmi G. Ayelagbe; Idris N. Abdullahi; Ibrahim Munirudeen
    ABSTRACT Objective: This study investigates the role of Apoptotic Protease Activating Factor-1 (APAF-1) in CD4+ cell depletion among human immunodeficiency virus (HIV) patients. Materials and Methods: This is a cross-sectional study in which 105 participants were enrolled, including 60 confirmed HIV-positive patients and 45 HIV-negative controls. HIV-positive patients were further divided based on CD4+ cell counts: Group 1 (<200), Group 2 (200–499), and Group 3 (≥500). An enzyme-linked immunoassay was used to measure APAF-1 levels, and CD4+ T-cell counts were enumerated using a Cyflow counter. Independent student’s t-test, Kruskal-Wallis, and Spearman’s correlation were utilized as needed. Results: Results showed significant reductions in lymphocytes, platelets, red blood cells, hemoglobin, albumin, and CD4+ cell values among HIV-infected individuals compared to controls. Conversely, APAF-1 and total protein levels were elevated in HIV-positive patients. Among HIV-positive groups, those with CD4+ cell counts <200 exhibited the highest median serum APAF-1 concentration. However, these differences were not statistically significant when compared with the other seropositive groups with CD4+ cell counts between 200 and 499 (P = 0.6726) and CD4+ cell counts of 500 or greater (P = 0.4325). The control group had the lowest median SAPAF-1 concentration, significantly different from HIV-positive groups. Positive correlations were observed between CD4+ counts and lymphocytes, hemoglobin, and hypoalbuminemia, while negative correlations were found between these parameters and APAF-1 levels. Conclusion: APAF-1 is a host factor that potentially contributes to CD4+ cell depletion. Similarly, APAF-1, serum total protein, and albumin levels were found to be predictive of disease progression and could serve as valuable diagnostic biomarkers in the monitoring of HIV/AIDS. Keywords: Apoptotic protease activating factor-1, CD4+ T cell, cytopenia, human immunodeficiency virus/acquired immune deficiency syndrome, hypoalbumin, progressor