Browsing by Author "Bamidele Oluwarotimi Omitoyin"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Composition of Defatted and Non-Defatted Black Soldier Fly Larvae Meal: Alternative for Fish Feeds
    (Direct Research Journal of Agriculture and Food Science, 2025-04) Kafayat Aina Iyapo; Bamidele Oluwarotimi Omitoyin; Emmanuel Kolawole Ajani; Oladimeji Kazeem Kareem; Adeyemo Muniru Adewole
    Black soldier fly (Hermetia illucens) larvae meal has been considered as a great source of animal protein for animals. Nutrient composition of the larvae varies according to the substrate used in rearing the larvae and the processing techniques. This study was conducted to investigate the nutrient composition of full-fatted and processed (defatted and non-defatted) black soldier fly larvae meal. The larvae reared on wheat bran substrate were collected from Food and Agriculture Organization, University of Ibadan (FAO/UI) black soldier fly larvae culturing unit, sacrificed with hot water, rinsed and divided into full-fatted and processed (defatted and non defatted) groups. The defatted group was mechanically pressed by squeezer to remove the fat content and the non-defatted group was not contacted. Both defatted and non-defatted groups were independently subjected to oven and sundried procedures. Chemical compositions of the full-fatted larvae meal, defatted oven-dried larvae, defatted sun-dried larvae, non-defatted oven-dried larvae and non-defatted sun-dried larvae were determined and statistically analysed. The research revealed black soldier fly larvae meal as a viable alternative to use as an animal protein source in fish feed formulation. Additionally, the nutritional composition revealed sun-dried processing method as the best method to remove the black soldier fly larvae lipid.
  • No Thumbnail Available
    Item
    Microbial assessment of Clarias gariepinus fillets fed defatted and non-defatted sundried black soldier fly larvae meal
    (2025-08) Kafayat Aina Iyapo; Bamidele Oluwarotimi Omitoyin; Emmanuel Kolawole Ajani; Oladeji Kazeem Kareem
    Aquaculture production in Nigeria is hindered by the dependence on fishmeal as the main protein source in fish feed. Consequently, there is a need to explore alternative protein sources that is cost-effective, sustainable and pose no risk to consumers. This study evaluated the microbial loads and isolates in Clarias gariepinus fillets fed defatted (DF) and non-defatted (NDF) sundried black soldier fly larvae meal (BSFLM) diets over a period of 21 storage days with assessments at day 0, 7th, 14th and 21st, after 168 days of feeding trials. Fishmeal was replaced with DF and NDF sundried BSFLM at varying inclusion levels of 0%, 25%, 50%, 75% and 100% in the diets of Clarias gariepinus, which were fed for a period of 168 days. Following the feeding trial, the fish were saline-rinsed, filleted, and sealed in double polyethylene bags. The fillets underwent boiling for 20 minutes, after which the fish were foil-wrapped, grouped in ziplock bags, labelled and stored at 4°C for 21 days in a chest freezer. Microbial analysis was estimated for each labelled ziplock bag sample at days 0, 7th, 14th, and 21st of the storage period, assessing total bacterial count (TBC), total coliform count (TCC), mould yeast, and microbe isolates. The results obtained showed that at day zero of storage, the highest TBC (8.0 x 105 CFU/g) was observed in fillets fed 75% defatted BSFLM, with Bacillus and Candida spp. dominating the isolates. At seven days of storage, the TBC ranged from 1.02 x102 CFU/g (0% defatted BSFLM) to 9.6 x105 CFU/g (75% defatted BSFLM), with Staphylococcus, Bacillus, and Candida spp. being the most persistent isolates. At the twenty-first day of storage, TBC in fish fillets fed 0% and 25% defatted BSFLM were more than 300 colony counts, which were recorded as too numerous to count because they exceeded permissible limits for consumption, and a broader spectrum of microorganisms, including Staphylococcus spp., Bacillus, Aspergillus spp., and Klebsiella spp., were isolated. This study concluded that catfish fillets fed defatted and non-defatted sundried BSFLM diets should be consumed within a shorter storage period due to the potential microbial risks associated with prolonged storage.