Browsing by Author "Adedayo, M.R."

Now showing 1 - 5 of 5
  • Thumbnail Image
    Item
    ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF Adansonia digitata STEM BARK EXTRACT ON SOME CLINICAL ISOLATES
    (Obafemi Awolowo University, Ile Ife, 2020) Ajiboye, A.E.; Sadiq, S.O.; Adedayo, M.R.
    Adansonia digitata is a massive and deciduous tree with a height of about 24 m and of significant economic importance. The antimicrobial and phytochemical screening of the aqueous and ethyl-acetate extract of stem bark of the plant were determined on some clinical isolates. The stem bark of the plant was collected and washed o properly before drying at 28 C. The pulverised stem bark was extracted with water and ethyl-acetate and screened for phytochemicals (qualitative and quantitative) using standard methods. The clinical isolates used were identified as Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli and Candida albicans. The antimicrobial activities of the crude extracts were carried out using the agar well diffusion methods. The minimum inhibitory concentration (MIC), minimum bactericidal and fungicidal concentrations were carried out using standard methods. The aqueous extract exhibited a higher zone of inhibition against S. aureus (14.00 ± 0.57 mm) at a concentration of 200 mg/ml while a zone of inhibition of 11.66±0.33 mm was observed for E. coli using ethyl acetate extract. Candida albicans had a zone of inhibition of 11.66±0.88 mmand 11.00±0.57 mm using aqueous and ethyl-acetate extracts respectively at 200 mg/ml. The MIC was 200 mg/ml for the crude extracts against the clinical isolates. The qualitative ethyl-acetate phytochemical screening revealed the presence of phenol, flavonoids, tannins, alkaloids, saponin and terpenoids. Phenol had the highest concentration of 2.02±0.25 mg/ml while terpenoids had a value of 1.38±0.02 mg/ml. Aqueous and ethyl-acetate extract of A. digitata possess significant antimicrobial activity against E. coli, S. aureus and C. albicans. However, K. pneumoniae showed resistance to ethyl acetate extract
  • Thumbnail Image
    Item
    Beta-Lactamase production and antibiotic susceptibility screening of Staphylococus aureus isolated from ready to eat fruits sold in some parts of Offa Metropolis, Nigeria
    (2024) Adedayo, M.R.; Emmanuel, T.O.; Ajiboye, A.E.
    The global menace of community-acquired antibiotic resistance of Beta Lactamase-producing Staphylococcus aureus has been traced to the increased consumption of Ready-to-eat Foods/Fruits. Samples each of ready-to-eat whole and sliced fruits (sliced pawpaw, apple, sliced watermelon, garden egg, cucumber, pear, guava, sliced coconut, berry and date fruit) were collected randomly from vendors in Offa, Kwara State, in Nigeria. Isolation and characterization of Staphylococcus aureus from the samples were done. The isolates were screened for Beta-Lactamase production and susceptibility to some antibiotics using standard microbiological techniques. A total of twenty-two (Twenty coagulase-positive and two coagulase-negative) Staphylococcus aureus was isolated. The total Staphylococal count was highest in sliced pawpaw (23.30 ± 2.75 × 10⁵ cfu/g) while the least was recorded in apple (3.0 ± 0.01 × 10⁵ cfu /g). Twenty (20) isolates were recorded to be Beta Lactamase producers. All the Beta Lactamase producers were 100 % resistant to Aztreoname, 80 % to Amoxicillin Clavulate, 45 and 35 % to Ceftazidine and Ceftriaxone. Thirty percent of the isolates were found to be susceptible to Ceftazidine only while 25 % were susceptible to Ceftriaxone only. The study concluded that increased incidence of Community-Acquired Multidrug-Resistant Staphylococcus aureus could be traceable to the consumption of unhygienic processed Ready-to eat Fruits. The ripple effects could be dangerous to human health.
  • Thumbnail Image
    Item
    Isolation, Identification and Screening of Humic Acid Producing Fungi from Soil Environment of Oil palm (Elaeis guineensis) Associated with Empty Fruit Bunches
    (Department of Microbiology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Kastina., 2023-12) Kawata, Hassan Musa; Omojasola, P. F.; Ajiboye A.E .; Adedayo, M.R.; Bale, Sheriffdeen Issa
    The demand for sustainable and sustainable biotechnological processes has developed as a result of a growing interest in converting agricultural waste into valuable products. In this study, fungal strains were isolated from soil environment of oil palm empty fruit bunches and screened for humic acid production. In Osun state, Nigeria, soil sample was taken from a local palm oil mill. Different colonies of fungi were obtained for further research using the serial dilution plating procedure. Plate screening was used to evaluate the fungal biomass as well as colony diameter of the fungal isolates, and a submerged fermentation test was used to measure the amount of humic acid concentration the isolates produced. Fungal isolates were identified by using molecular methods and morphological analysis. The fungi species Aspergillus niger, Rhizopus stolonifer, and Penicillium chrysogenum were isolated from the soil environment. When compared to Rhizopus stolonifer, Aspergillus niger grew more superiorly in terms of zone diameter (26.5 1.50 mm) and biomass (2.23 0.16 g), while Penicillium chrysogenum showed no growth. Throughout the fermentation process, Aspergillus niger also produced a greater quantity of humic acid, with concentrations varying from 0.70 mg/l to 2.20 mg/l. Aspergillus niger strain HR18's existence was confirmed by molecular analysis. This experiment proved that Aspergillus niger can efficiently produce humic acid from OPEFB, providing a useful method for waste utilization and sustainable practices.
  • Thumbnail Image
    Item
    Itaconic acid production by Aspergillus terreus using pod of Moringa oleifera as substrate in solid state fermentation.
    (Nigerian Society of Microbiology, 2018) Ajiboye, A.E.; Babatunde, S.K.; Adedayo, M.R.; Ajuwon, I.B.; labinjo, Z.Y
    Itaconic acid was produced by Aspergillus terreus under solid state fermentation using the pod of Moringa oleifera as substrate. Grinded Moringa pod was weighed into seperate shake flasks containing salts and Aspergillus terreus (3.2 x 104 spores/ml ) was added. The substrate was left to ferment for 5 days. Carboxylmethylcellulose (CMC) was used as control substrate. Itaconic acid was assayed using standard procedures. Different fermentation parameters such as substrate concentration, inoculum size and incubation period were varied to determine the maximum production of itaconic acid. Light spectrophotometer was used to measure the absorbance of the itaconic acid concentration produced after each day of fermentation at 385nm. The maximum yield of itaconic acid of 18.39mg/ml was obtained with a substrate concentration of 10g while 17.2mg/ml was produced with an inoculum size of 6ml (3.2 x 104 cfu/ml ) and 13.91mg/ml on day 5. It is concluded that the pod of Moringa oleifera can be used as a substrate for itaconic acid production using A. terreus in solid state fermentation.
  • Thumbnail Image
    Item
    Mycoflora of Phoenix Dactylifera (Date Palm) Fruit Sold in Some Markets in Ilorin
    (Globe edu Group www.theijst.com, 2016) Adedayo, M.R.; Babatunde, S.K.; Ajiboye, A.E.; Adetuyi, E.O.
    Aim: the research was conducted to isolate and characterize spoilage mycoflora present on dried date palm fruit commonly sold in some markets within Ilorin metropolis. Method: samples were collected from eight different market locations within Ilorin metropolis. The samples were surface sterilized and split opened to isolate fungi from them and the isolates were characterized morphologically on plate and under the microscope. Characteristics observed were compared with literature for the identification of the specimen following standard methods. Physicochemical analysis was also conducted on the samples according to standard techniques. Result: A total number of six fungi were isolated and identified as Apergillus niger, Neurospora crasa, Aspergillus fumigatus, Aspergillusflavus, Penicillum chrysogenum, and Syncaehalastron racemosus withA. niger having the highest percentage of occurrence. The physicochemical analysis revealed that the samples has high percentage of sugar and mineral contents. Conclusion:the study revealed that date palm fruit sold within Ilorin metropolis is heavily contaminated with fungi. The fungi responsible for its spoilage are those that have been known to produce toxic substances which have adverse effect on the consumer’s health.