Department of Microbiology

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Protease Production From the Pod of African Parkia biglobosa Using Resident Fungi in Solid State Fermentation
(2017) Adedayo et al 2017
Protease production from the pod of naturally fermenting African Parkia biglobosa by bioprocess is a good value added to agro industrial residues. The production of enzyme protease by fungiwas carried out under solid state fermentation (SSF). The effects ofincubation period, pH of the medium and addition of supplements onprotease production (enzyme activity) by Aspergillus niger and Rhizopus stolonifer were determined. The optimal values of factors influencing the production were determined during theincubation period of 5 days. The enzyme activity increased until day three before it began to decline in both supplemented and non-supplemented fermented substrate at 28 ± 20C. The pH range for enzyme production was 6.0 – 7.0. Protease production was 1% enhanced when supplemented with nitrogen source(1% ammonium nitrate) but was not enhanced when supplemented with carbon source(1% maltose). In conclusion, production of protease under SSF using Parkia biglobosa pod as substrate is effective and could be encouraged in industrial processes like food processing, baking and other related processes.