Department of Microbiology

Permanent URI for this community

https://kwasuspace.kwasu.edu.ng/handle/123456789/115

Browse

Browsing Department of Microbiology by Author "1Ajiboye Adeyinka Elizabeth, 1* Said Olaitan Rukayat, 1Yusuf Monsurat Oluwabunmi"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Screening of Naturally Occurring Fungi for Lipase Production using Selected Agricultural Waste Products
    (Dutse Journal of Pure and Applied Sciences (DUJOPAS, 2025-06) 1Ajiboye Adeyinka Elizabeth, 1* Said Olaitan Rukayat, 1Yusuf Monsurat Oluwabunmi
    The growing industrial demand for new sources of lipases with varying catalytic properties has prompted the isolation and selection of novel microbial strains. This study focused on isolating and screening fungi that produce lipase using selected agricultural waste products. The farm waste materials which include; wheat bran, rice husks, corn meal, and sugarcane bagasse, were collected aseptically from selected markets. They were sun-dried for 72 hours and pulverized. Naturally occurring fungi were obtained using the solid-state fermentation method. Isolation and identification of the fungi were performed using the dilution plate method and characterization respectively. Lipase production was screened using a medium containing a phenol red indicator. Five fungal isolates were obtained from wheat bran, two from rice husks, two from corn meal, and one from sugarcane bagasse. Out of these, four fungal isolates tested positive for lipase production, while one tested negative. From wheat bran the highest zone of colouration was observed in Aspergillus niger with 41.00±2.65, Aspergillus flavus with 34.00±12.29, Rhizopus stolonifer with 29.00±5.29, Geotrichum species with 24.33±7.09 and Fusarium species with 0.00±0.00. In rice husk, the highest zone of colouration was observed in Rhizopus stolonifera with 85.00±0.00 Aspergillus niger with 83.67±1.53, in corn meal the highest zone of colouration was observed in Aspergillus flavus with 85.00±0.00, Rhizopus stolonifer with 42.34±16.19. In sugar cane bagasse, the highest zone of colouration was observed in Rhizopus stolonifer with 85.00±0.00. It can be concluded that wheat bran, rice husk, corn meal, and sugarcane bagasse are potential substrates for lipase production using naturally occurring fungi.