Antimicrobial Effects of Ocimum gratissimum Extracts on the Spoilage Organisms Isolated from Yoghurt Samples

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dc.contributor.authorAmupitan Adefunke Olawumi
dc.contributor.authorAjiboyeAdeyinka Elizabeth
dc.contributor.authorAdedayo Majekodunmi Rachael
dc.contributor.authorAmupitan Adewale Alex
dc.date.accessioned2026-05-14T10:28:37Z
dc.date.available2026-05-14T10:28:37Z
dc.date.issued2025-06-14
dc.descriptionThis research work is to assess the antimicrobial effects of the leaf extract of the plant Ocimum gratissimum on the Spoilage Organisms Isolated from Yoghurt Samples.
dc.description.abstractOcimum gratissimum (OG) is recognised for its nutritional, antioxidant, and antimicrobial properties, making it a strong candidate for natural food preservation. This study evaluated the effects of ethanolic and aqueous OG leaf extracts against spoilage organisms isolated from laboratory-prepared yoghurt samples stored at room temperature for ten days. The agar well diffusion technique was employed for antimicrobial screening, while the broth microdilution method was used to determine both minimum inhibitory concentration (MIC) and minimum cidal concentration (MCC). The isolated organisms included Bacillus subtilis, Bacillus spp., Saccharomyces cerevisiae, Saccharomyces bulderi, Pichia kudriavzevii, Aspergillus niger, and Aspergillus flavus, identified by standard microbiological methods. The aqueous extract at a concentration as low as 62.50 mg/mL inhibited Bacillus subtilis, Saccharomyces bulderi, Pichia kudriavzevii, and Saccharomyces cerevisiae, with inhibition zones ranging from 5.00±0.27 to 6.20±0.87 mm. The ethanolic extract exhibited antimicrobial effects against Bacillus subtilis, Bacillus spp., Saccharomyces bulderi, Pichia kudriavzevii, and Saccharomyces cerevisiae at the same concentration (62.50 mg/mL), but with larger inhibition zones ranging from 5.30±0.45 to 11.35±2.10 mm. The aqueous extract showed substantial inhibition of Bacillus subtilis, Saccharomyces bulderi, and Pichia kudriavzevii, with an MIC of 62.50 mg/mL. In contrast, the ethanolic extract demonstrated an MIC of 31. 25 mg/mL for Bacillus subtilis, Bacillus spp., and Aspergillus niger. The MCC of the aqueous extract was 250 mg/mL for Bacillus subtilis, Saccharomyces bulderi, Pichia kudriavzevii, Aspergillus flavus, and Saccharomyces cerevisiae, while the MCC of the ethanolic extract was 125 mg/mL for Bacillus subtilis and Bacillus spp. Overall, the OG extracts exhibited substantial antimicrobial effects, effectively inhibiting the growth of spoilage organisms isolated from stored yoghurt samples. Harnessing the potential of OG can lead to an extended shelf life for yoghurt, positioning OG as a promising natural preservative
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dc.identifier.citation1
dc.identifier.issnE-ISSN: 2814 – 1822; P-ISSN: 2616 – 0668
dc.identifier.urihttps://kwasuspace.kwasu.edu.ng/handle/123456789/7123
dc.language.isoen
dc.publisherUJMR
dc.titleAntimicrobial Effects of Ocimum gratissimum Extracts on the Spoilage Organisms Isolated from Yoghurt Samples
dc.typeArticle
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This research is to assess the extract of the leaf of Ocimum gratissimum Plant on the spoilage organisms that were isolated from yoghurt
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