HAEMATOLOGICAL RESPONSE OF BROILER CHICKENS TO BIO-CONTROL METHOD OF AFLATOXIN MITIGATION
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Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Wayamba University sri lamka
Abstract
Aflatoxin is toxic, carcinogenic and
ubiquitous in nature affecting both crops and
livestock. Mitigating aflatoxin effect using
toxin binders has not been very effective.
Information on the use of biological methods
in aflatoxin mitigation has not been
adequately documented. Therefore, effect of
aflatoxin bio-control method (Aflasafe) on
growth indices, haematology and serum
biochemistry of broiler chickens (BC) was
investigated. One-day old Marshal BC
(n=1020) were allotted to four treatments:
Aflasafe maize-based diet (AMBD), farm
feed (FF), aflatoxin-contaminated diet with
toxin binder (ACDTB) and aflatoxin contaminated diet without toxin binder
(ACDWTB). The contaminated diets
contained 306.3ppb aflatoxin and the
experimental design was completely
randomised with five replicates (n=255) per
treatment for 8 weeks. Blood (5mL) was
collected at 8th week to determine the
erythrocytes, packed cell volume (PCV),
Haemoglobin (Hb), eosinophil, basophil,
Mean Corpuscular Volume (MCV), Mean
Corpuscular Haemoglobin (MCH). Data
were analysed using descriptive statistics
and ANOVA at α0.05. The highest value
(28.08±0.61%) for PCV at the starter phase
was obtained in birds on treatment 3 fed
ACDTB, while the least value
(27.16±0.34%) was recorded in birds on
treatment 1 fed AMBD. Birds fed the FF+
toxin binder (T2) had the highest Hb value
(9.11±0.17g/dl), while the birds fed AMBD
had the least value (8.67±0.11g/dl).
However, at the finisher phase, PCV of birds
fed (T3) was significantly (P˂0.05) higher
(29.06±0.72%) than that of birds fed the
AMBD (26.88±0.79%). The erythrocyte
count (3.90±0.12x106
) of broiler chickensfed the ACDWTB (T3) was observed to be
significantly (P˂0.05) higher than the values
obtained in birds fed the AMBD
(3.30±0.34x106
/l). The mean value recorded
for eosinophils in birds fed the ACDWTB
(2.00±1.2%) was significantly (P˂0.05)
lower than that of the birds fed the FF
(3.16±1.72%) which contained toxin binder
(Control diet). Basophil values (0.20±0.5%,
0.83±1.4% and 0.32±0.6%) of birds fed
AMBD (T1), ACDTB (T3) and ACDWTB
(T4) respectively were not significantly
different from that of the control value
(0.32±0.8%). Birds fed ACDTB
(7.92±0.85%) had a significantly (P˂0.05)
lower MCV value compared with the value
(8.95±0.98%) obtained from the control diet
(FF+ Toxin binder).