Investigation on the hydrocarbon utilization potential of Pseudomonas putrefaciens, Bacillus stearothermophilus and Streptococcus faecium isolated from crude oil contaminated soil
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Date
2011-12-19
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Publisher
PELAGIA RESEARCH LIBRARY
Abstract
The biodegradation potential of Pseudomonas putrefaciens, Bacillus stearothermophilus and
Streptococcus faecium isolated from crude oil contaminated soil was investigated in this
study. The 3 bacteria strains were inoculated into crude oil supplemented with Mineral Salt
Medium and incubated at 37o C for 25 days. The results obtained showed that P. putrefaciens, B.
stearothermophilus and S. faecium were able to degrade 70%, 58% and 48% at the end of 25
days of incubation respectively. IR analysis of the residual crude oil was done, the results
obtained showed that the bacteria strains have exerted microbial action on the crude oil. The
results obtained from IR analysis revealed 17 bands, 16 bands and 12 bands for P. putrefaciens,
B. stearothermophilus and S. faecium respectively, while control had 10 bands. This is an
indication that the bacteria strains have modified the crude oil constituents. P. putrefaciens had
highest percentage of degradation compare to the other two bacteria. The order of degradation
was as follows P. putrefaciens > B. stearothermophilus > S. faecium. This study showed that
the bacteria strains used in this study could be relevant in the bioremediation of ecosystem that
may be contaminated with hydrocarbons.
Description
Oil spills are an inevitable consequence of using petroleum.Spills can occur at the oil refinery,
during transportation, natural seeps or during routine maintenance of infrastructure (Ainon et al.,
2010).These oil spills devastate the soil surface, and underground water and alter the microbial
population at the polluted sites.
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Citation
opasola et al., 2011 European Journal of Experimental Biology, 2011, 1 (2):189-197