Biosynthesis of Ag and TiO2 nanoparticles and the evaluation of their antibacterial activities
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Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
Inorganic Chemistry Communications
Abstract
In this study, Pseudomonas aeruginosa was used for the synthesis of silver and titanium oxide nanoparticles (Ag and TiO2 NPs). The nanoparticles were prepared by mixing the supernatant solution of the cultured organism and the solution of silver nitrate and titanium tetrabutoxide. UV–Vis spectroscopic analysis gave absorption peak at wavelength of 414 nm and 390 nm for Ag and TiO2 NPs respectively. The FTIR spectra gave absorption bands attributed to Nsingle bondH stretch of the peptide linkages, Osingle bondH stretch of carboxylic acid, amide bend, and single bondCdouble bondO stretch which may have emanated from the micro-organism. The powdered X-ray diffraction (PXRD) studies revealed the crystalline nature of AgNPs and TiO2 NPs while microscopic studies showed spherical and dispersed morphologies with average particle size of 7.27 nm and 6.83 nm for Ag and TiO2 NPs respectively. Antibacterial analysis of the synthesized nanoparticles showed broad spectrum inhibition against disease causing microorganisms with TiO2NPs exhibiting excellent results more than AgNPs and the control drugs.