STUDIES ON METAL- RESISTANT BACTERIA ISOLATED FROM SPENT- ENGINE OIL CONTAMINATED SOIL, IN OGBOMOSO, OYO STATE, NIGERIA.
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Date
2015-05-20
Journal Title
Journal ISSN
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Publisher
International Journal of Research in Environmental Science and Technology Universal Research Publications.
Abstract
This study was carried out to establish the relationship between hydrocarbon utilization potential and heavy metal tolerance
in bacteria species. Nineteen bacteria species were isolated from soil samples collected from four (4) automobile repair
workshops in Ogbomosho, Oyo state Nigeria.The bacteria isolated were identified to be Klebsiella oxytoca(5), Klebsiella
edwardsii(13)and Shigella alcalescens(1). Metal tolerance assay revealed that Klebsiella oxytoca, Klebsiella edwardsii and
Shigella alcalescens have the ability to grow in the presence of Zncl2, Pbcl2 and CuSO4. The minimum inhibitory
concentration (MIC) of the metal salts ranged from 1mM to >16mM for Pbcl2, 2mM to >16mM for Zncl2 and 8mM to >24
for CuSO4. The ability of the bacteria strains to utilize hydrocarbons was also investigated by measuring the optical density
of the strains in minimal salt mediumsupplemented crude oil at 625nm. The results showed that there is relationship
between hydrocarbon utilization and heavy metal tolerance among the bacteria.
Keywords:
Description
Environmental pollution is a global problem because of its
adverse effects on human beings, plants and animals.
Heavy metals are increasingly found in our environment
due to natural and industrial process. It has been well
reported that microbial interactions with metals may have
several implications for the environment such that microbes
can be used in the biogeochemical cycling of toxic heavy
metals as well as in cleaning up or remediating metal
contaminated environments (Badar et al., 2000). Salt et al.,
(1998) also reported that bacteria tolerance to heavy metals
can be used to minimize the effects of heavy metals on total
biological activity of the ecosystem. Processes involved in
reducing metal toxicity are of considerable current interest
particularly for highly contaminated heavy soils.
Keywords
Citation
Opasola et al., (2015), STUDIES ON METAL- RESISTANT BACTERIA ISOLATED FROM SPENT- ENGINE OIL CONTAMINATED SOIL, IN OGBOMOSO, OYO STATE, NIGERIA. International Journal of Research in Environmental Science and Technology 2015; 5(2): 62-67