Browsing by Author "OlugbemiT.Olaniyan"

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    QuercetinmodulatesgranulosacellmRNA androgenreceptorgeneexpressionin dehydroepiandrosterone-inducedpolycystic ovaryinWistarratsviametabolicandhormonal pathways
    (Journal ofBasicandClinicalPhysiologyandPharmacology - DE GRUYTER, 2019) OlugbemiT.Olaniyan
    Background: It is estimated that about 5–10% of women suffer from polycystic ovarian syndrome (PCOS) which is a major cause of female reproductive dysfunction. This study examined the role of quercetin on de hydroepiandrosterone (DHEA)-induced PCO in Wistar rats. AutomaticallygeneratedroughPDFbyProofCheckfromRiverValleyTechnologiesLtd Methods: Twenty-eight pre-pubertal female Wistar rats that are 21 days old weighing 16–21 g were sorted into four groups (n = 7). Group I served as control and was given distilled water only, Group II were injected with 6 mg/100gBWofDHEAin0.2mLofcornoilsubcutaneously, Group III received 100 mg/kg BW of quercetin orally and Group IV received 6 mg/100 g BW of DHEA in 0.2 mL of corn oil subcutaneously and 100 mg/kg BWof quercetin orally. Rats were sacrificed after 15 days by cervical dislocation method. Blood samples and ovaries were collected for hormonal, biochemical, and histopathological analysis and expressions of mRNA androgen receptor gene were determined using RT–qPCR. All data were analysed using one-way ANOVA. Results: Asignificant decrease (p <0.05) intheantioxidant andmetabolicenzymeactivityintheDHEAtreated group was observed when compared with control. DHEA co-administration with quercetin showed a signifi cant decrease in malondialdehyde and cytokines when comparedwithDHEAtreatedgroup.Alsoasignificant increase in progesterone,metabolicandantioxidantenzymeactivitywasobserved.Thehistopathologydemon strates a reduction in cystic and atretic cells, improved expression of BCl2, E-Cadherin and a decrease in Bax. Conclusions: Quercetin alleviated DHEA-induced PCO. These effects could be attributed to its antioxidant property.