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  1. Home
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Browsing by Author "Olayinka Abosede Ojo"

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    Antioxidant Potentials of Parquetina nigrescens Leaf Extract Administration in Broiler Chicken Production
    (Pharmaceutical Sciences, 2023) Adeyinka Oye Akintunde; Lois Chidinma Ndubuisi-Ogbonna; Ayomide Sobowale; Herman Enericheckor Irorevbo; Olayinka Abosede Ojo; Samson O. Oyewumi; Bolatito Adenike Shobo; Olufunso Emmanuel Akinboye; Elizabeth Oluwafunmiso Ngozi
    This study looked into the grill chickens' Parquetina nigrescens leaf extract (PNLE) antioxidant capacity. Parquetina nigrescens leaves that were still fresh were collected from Ilishan-Remo in Ogun State, Nigeria. The experiment was carried out in the Babcock University Farmhouse's chicken department in Ilishan-Remo, Ogun State, Nigeria. After gathering the leaves and combining them with 50g of the leaves in 1000ml of water, the extract was produced. A total of 200-day-old Ross broiler chicks were divided into five groups at random and given different amounts of PNLE in 500 millilitres of water (0, 0.2, 0.4, 0.6, and 0.8 millilitres). The experiment lasted for 42 days. The design of the experiment was completely randomized design. Data was collected on performance, hematology, serum biochemical and antioxidant parameters, and temperature and humidity were monitored regularly. Data were subjected to a one-way analysis of variance (p<0.05). There was no significant difference (p>0.05) in performance characteristics. There was a significant difference (p<0.05) in malonaldehyde with the 0ml PNLE group having the highest value (3.5U/L X 109). There was a significant difference in catalase and superoxide dismutase with the 0.4ml PNLE group having significantly highest (p<0.05) values. Glutathione peroxidase was considerably (p<0.05) impacted by PNLE administration. Total protein, albumin, globulin, glucose, cholesterol, triglycerides, urea, aspartate transaminase, and alanine transaminase were not substantially (p>0.05) affected by PNLE delivery. The control group had considerably lower (p<0.05) values for alanine phosphatase and red blood cell counts, and significantly higher (p<0.05) values for creatinine. It is concluded that PNLE has good antioxidant potential thus improving the health status of broiler chickens. Antioxidants, Oxidative stress, Parquetina nigrescens, Ross broilers
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    Biological Control of Aflatoxin on Egg Production Performance of Laying Hens
    (Sciencedomain international, 2020) Olayinka Abosede Ojo; Omololu Olusanya
    The deleterious effects of aflatoxin have been well documented in the literature on livestock and animal products. Although toxin binders and some methods of mitigating aflatoxin has been proven to illicit a positive response, but the effectiveness of biocontrol method of mitigation on the laying performance, egg production and characteristics have not been fully documented. Therefore, effect of aflatoxin bio-control method (Aflasafe) on growth indices, egg production and characteristics of laying chicken (LC) were investigated in this study. 700 point-of-lay Bovan Nera (LC) were randomly allotted to four dietary treatments: Aflasafe maize-based diet (AMBD), Farm Feed with toxin binder (FF+toxin binder), aflatoxin-contaminated diet with toxin binder (ACDTB) and aflatoxin contaminated diet without toxin binder (ACDWTB). The contaminated diets contained 306.3ppb aflatoxin and the experimental design was completely randomised with 4 treatments (n=175) and 5 replicates (n=35) per treatment for 14 weeks. Daily feed intake (DFI), Average egg weight (AEW), Average egg width (AEWd), Albumen length (AL) were determined using standard procedures. The DFI (g) of LC on ACDTB (113.54) and ACDWTB (115.13) was significantly lower than AMBD (124.66) and FF+toxin binder (129.06). The AMBD (1.45 g) significantly enhanced the AYD than other treatments. Aflasafe maize-based diet enhanced, egg characteristics in laying chicken. It also improved egg quality in laying chickens without adverse effect on the production. The use of aflasafe maize grain in poultry diet is recommended
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    Effect of dietary cinnamon supplementation on the growth in length, haematology, serum biochemistry, intestinal microbiota and enzymes and histomorphological changes of the intestine, liver and the kidney of Heteroclarias
    (Faculty of Veterinary Medicine at Chiang Mai University, 2023) Wasiu Adeyemi Jimoh; Ayodeji Ahmed Ayeloja; Olayinka Abosede Ojo; Comfort Timileyin Ayodele; Adijat Ebunlomo Alabi; Grace Amara Obinnakwelu
    In a 56-day feeding trial, the impact of dietary cinnamon supplementation on juvenile Heteroclarias (Clarias gariepinus ♀ × Heterobranchus bidorsalis ♂) was examined. Five experimental diets with varying levels of cinnamon powder (ranging from 0% to 2.0%) were formulated. The study found that cinnamon supplementation positively influenced the fish's growth, haematology, serum biochemistry, intestinal microbiota, and enzyme activities. The group fed with 1.5% cinnamon (DT4) showed the highest growth performance, haematological indices, antioxidant, and hindgut enzyme activities. Additionally, the cinnamon-fed groups exhibited a significant reduction in total cholesterol levels, except for the 0.5% cinnamon-fed group (DT2). Blood glucose, blood urea nitrogen, and creatinine levels also decreased significantly in the cinnamon-fed groups compared to the control. Cinnamon supplementation increased bacterial counts, but fungi counts showed diverse effects, with the most favourable diversity indices observed in the 1.5% cinnamon group (DT4). Amylase, cellulase, and trypsin activity in the cinnamon-fed group were significantly higher than in the control group. Notably, all cinnamon-fed groups' intestine, liver, and kidney microstructures remained well-preserved, without any signs of injury. This study demonstrates that cinnamon has the potential to enhance the growth and overall well-being of African catfish hybrids. The optimal outcomes were observed when cinnamon was supplemented at a level of 1.5%, which can have significant implications for improving aquaculture practices and fish health
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    Effect of phytogenic supplements on the reproductive physiology and metabolic hormones of rabbits exposed to heat stress conditions
    (Elsevier, 2023) Olatunji Abubakar Jimoh,; Olajumoke Temidayo Daramola; Hafsat Ololade Okin-Aminu; Olayinka Abosede Ojo; Wahab Adekunle Oyeyemi
    This study investigated the role of phytogenic supplements on the reproductive physiology and metabolic hor mones of rabbits exposed to heat stress conditions. Fresh Moringa oleifera, Phyllanthus amarus and Viscum album leaves were obtained and processed into a leaf meal using standard procedure and served as a phytogenic supplements. Eighty rabbit bucks of 6 weeks old (514.84 ± 14.10 g) were randomly assigned to the four dietary groups consisting of Diet 1 without the leaf meal (control diet) and Diets 2 (D2); 3 (D3) and 4 (D4) contained 10% Moringa, 10% Phyllanthus and 10% Mistletoe, respectively, in an 84-day feed trial at the peak of thermal discomfort. Semen kinetics and seminal oxidative status, reproductive and metabolic hormones were assessed using standard procedure. Results reveal that sperm concentration and motility traits of bucks on D2, D3 and D4 were significantly (p < 0.05) higher than bucks on D1. Spermatozoa speed traits of bucks on D4 were signifi cantly (p < 0.05) higher than bucks on other treatments. Seminal lipid peroxidation of bucks in D2-D4 was significantly (p < 0.05) lower than bucks on D1. Corticosterone of bucks on D1 was significantly higher than bucks on other treatments (D2-D4). Lutenizing hormone of bucks on D2 and testosterone of bucks on D3 was higher (p < 0.05) than in other groups, while follicle stimulating hormone of bucks on D2 and D3 were higher (p < 0.05) than bucks on D1 and D4. In conclusion, the three phytogenic supplements improved sex hormones, enhanced spermatozoa kinetics, viability and seminal oxidative stability of bucks during heat stress condition
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    EFFECTS OF ORAL INTAKE OF Mucuna pruriens SEED POWDER ON THE HEMATO-BIOCHEMICALAND REPRODUCTIVE PERFORMANCE OF BUCKS
    (Faculty of Agriculture at the University of Abuja, 2026) Okukpe Kehinde Matthias; Abdullahi Mariam Alafara; Olayinka Abosede Ojo; Chimezie Victoria Oluladun; Adeyina Adebisi Olusegun; Alli Oluwasayope Ibidapo; Ajao Babatunde Hadiyat; Familoni Olugbenga Michael
    The use of Mucuna pruriens seed extract for the treatment of male reproductive dysfunction has been well researched in animals and humans but the various methods of extractions and solvents used as well as the route of administration often influence the effectiveness of the plants seed. The experiment involve the use of fifteen West African Dwarf (WAD) bucks in a completely randomized design to study the effect of oral administration of Mucuna pruriens powder on the reproductive performance of WAD bucks. The bucks weighing between 9kg-10kg were randomly assigned to five treatments. The experiment lasted for 7weeks, during which body weight was taken weekly along with rectal temperature. Blood was collected fortnightly for haematological and biochemical analysis while semen was collected by electro-ejaculation at the end of the experiment for semen analysis. There was no significant difference (p>0.05) in all the haematological and biochemical parameters except with White blood cell, Red blood cell, Alkaline Phosphate and Cholesterol which were significantly higher with increase in Mucuna pruriens powder. There was significant difference (p < 0.05) in all the sperm characteristics such as the sperm motility (%), sperm count (x106 /ml), live to dead ratio, normal sperm morphology and semen volume (ml). Rectal temperature was normal in all the groups and weight gain was not significantly affected throughout the period. It was concluded that oral administration of 10g of Mucuna pruriens powder was the best in terms of improvement of semen characteristics of West African Dwarf buck
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    Effects of Tomato Juice Supplementation on the Reproductive Performance, Hematological Indices and Oxidative Status of Gestational Sows in Ilorin, Nigeria.
    (Badeggi Journal, 2023-05-01) Olayinka Abosede Ojo; David Awoniyi
    A ten-week trial was conducted to evaluate the efficacy of orally administered tomato juice (ToJ) on the hematological and reproductive performance of gestational sows raised at Omolara Farms, Ogbagba town, Kwara State. A Sixteen (n=16) gestational sows within 4 weeks of gestation were randomly allotted into four treatment groups, having four replicates, with one sow representing a replicate Both Treatment 1 and 2 received no ToJ, but each received only 60 mls of clean water, plus 1g of Vitamin C in treatment 2., Treatment 3 and Treatment 4 received ToJ at 60mls and 120 mls respectively. At 10 th th -6 week of experiment, blood samples were collected via marginal ear vein; hematological parameters evaluated include packed cell volume (PCV), red blood cell (RBC), hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), white blood cell (WBC) and platelet. Reproductive data monitored include; number of piglets per sow (NPPS), mortality rate, number of stillbirth piglets (NSBP), number of surviving piglets (NSP), number of piglets at birth (NPB). Result revealed that hemoglobin and WBC values were significantly depressed, when compared with the negative and positive controls (T1 and T2), while being significantly elevated in pregnant sows administered with 60mls and 120mls ToJ. NPPS, NPB and mortality rate were significantly elevated and an abysmal increase in NSBP at 60 mls ToJ, while the reduced values of these parameters were recorded at 120 mls ToJ, as compared to the values observed in sows given Vitamin C. Serum antioxidant enzymes were elevated at 120 ToJ. Therefore, its recommended that ToJ inclusion could enhance performance of pregnant sows up to 120 mls under tropical condition
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    Effects of turmeric, ginger, cinnamon, and garlic essential oils on HSP70, NFκB, oxidative DNA damage, inflammatory cytokines, and oxidative markers in broiler chickens
    (Oxford University Press, 2024) Olatunji Abubakar Jimoh; Ayoola Doris Ayodele; Olayinka Abosede Ojo; Hafsat Ololade Okin-Aminu; Olumuyiwa Joseph Olarotimi
    In recent years, the use of natural bioactive compounds derived from spices has garnered significant interest in poultry production due to their potential to modulate immune responses and oxidative stress. An investigation into the roles of spices essential oils (EO) on inflammatory cytokines, HSP70 and oxidative markers of broiler chickens was conducted in this study. Four spices consisting of garlic, ginger, turmeric, and cinnamon were processed to obtain their respective EO. Two hundred 1-d-old arbo acre broilers were allotted to 5 treatments consisting of B1 (control), B2 (garlic EO), B3 (ginger EO), B4 (Turmeric EO), and B5 (cinnamon EO), with EOs administered to drinking water at 30% (v/v) in a 49-d trial. Blood was sampled for assessment of hematological parameters, and serum obtained were assayed for inflammatory cytokines, anti oxidant activities, nuclear factor kappa B (NFκB), 8-hydroxydeoxyguanosine (8-OHdG), and heat shock protein 70 (HSP70) levels using standard procedures. Results obtained revealed that cinnamon EO enhanced erythrocytic indices, leukocyte profile, catalase, glutathione peroxidase and interleukin 10, lowers interleukin 1 beta (IL-1β) and interferon gamma (IFN-γ), enhanced HSP70 and higher 8-OHdG levels in chicken. Garlic EO enhanced monocytes and superoxide dismutase, while reduced IFN-γ and HSP70, but increased IL-1β and tumor necrosis factor alpha (TNF-α) NFκB in broiler chickens. Ginger EO also enhanced erythrocytic indices, total antioxidant activity, lowered IFN-γ and lipid peroxidation, while turmeric EO enhanced total antioxidant activity, catalase and lowered IFN-γ and increased 8ohdg in broiler chickens. In conclusion, this study revealed that ginger and turmeric EO were more beneficial in preventing oxidative DNA damage, cinnamon EO enhanced serum oxidative status and lowered pro-inflammatory cytokines, while garlic EO reduced HSP70 in broiler chickens.
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    Fenugreek Supplementation in Hybrid African Catfish Diets: Effect on Growth Performance, Physio-Metabolic Response, and Antioxidant Enzyme Capacity
    (Wiley, 2024) Wasiu Adeyemi Jimoh; Femi John Fawole; Ayodeji Ahmed Ayeloja; Olayinka Abosede Ojo; Boluwatife Mary Ijalaye; Aishat Yetunde Adesaogun; Emmanuel Oladele Olaitan
    The impact of dietary fenugreek (FN) supplementation on growth performance, physio-metabolic enzyme response, and antioxi dant enzyme activities in hybrid African catfish (Clarias gariepinus ♀ × Heterobranchus bidorsalis ♂) was investigated in this study. Five isonitrogenous diets (38% crude protein) were developed and fed to the fish for 60 days. The FN was incorporated into the diets at 0%, 0.5%, 1.0%, 1.5%, and 2%. Two hundred twenty-five fingerlings of hybrid African catfish (average weight 4.01 g Æ 0.02) were used for the study and randomly distributed to five dietary groups in triplicate. FN supplementation improved growth responses and survival rates; the dietary groups considerably (p<0:05) outperformed the control group. FN-based diets consider ably increased the carcass protein content (p ¼ 0:020) and improved protein retention (p ¼ 0:015). FN supplementation markedly (p<0:001) and dose-dependently reduced the carcass’s lipid content and substantially (p<0:001) increased the levels of metabolic enzymes. The fish antioxidant capacity was significantly enhanced (p<0:001), and hepatic stress enzymes were greatly (p<0:001) lowered in FN-based diets. In summary, the second-order polynomial regression analysis indicates that the optimal dose of FN supplementation to improve hybrid catfish growth without negatively affecting the fish’s physio-metabolic response or overall wellbeing is 1% dietary inclusion level.
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    HSP70, adiponectin, leptin, pro-inflammatory cytokines and metabolic hormones of heat-stressed broilers fed herbal supplements
    (Elsevier, 2023) Olatunji Abubakar Jimoh; Olajumoke Temidayo Daramola; Hafsat Ololade Okin-Aminu; Olayinka Abosede Ojo
    Global warming undoubtedly is a serious challenge facing poultry production especially in tropical climate, which subject animals to heat-stressed conditions leading to multiple physiological alterations. An investigation was conducted to determine the role of herbal supplements on adipokines, pro-inflammatory cytokines and metabolic hormones of broilers exposed to heat stress. 200 a day-old broiler chicks were randomly allotted to standard diets; diet 1 without leaf meal (T1), diet 2 with 5% Moringa oleifera (T2), diet 3 with 5% Phyllanthus amarus (T3) and diet 4 with 5% mistletoe; Viscum album (T4) in a 49-day feed trial during the peak of thermal discomfort in Southern Nigeria, to assess hormones, adipokines and cytokines using standard procedures. Results obtained shows that triiodothyronine of birds fed phyllanthus and mistletoe were significantly (p < 0.05) higher than birds on basal diet. Corticosterone of birds fed mistletoe and phyllanthus were significantly (p < 0.05) lower than those obtained in birds on basal diet. Heat shock protein of birds fed on moringa supplements were significantly (p < 0.05) lower than those in birds on basal diet. The adiponectin of birds fed on basal diet were statistically (p < 0.05) higher than other treatments. Interleukin 6 of birds on phyllanthus and mistletoe sup plements were not significantly (p > 0.05) different from birds on basal diet. Interleukin 1β of birds fed basal diet was significantly (p < 0.05) higher than birds fed moringa supplements and the significantly (p < 0.05) least values obtained in those of birds fed phyllanthus supplement. Tumor necrosis factor α of birds fed on phyllanthus and mistletoe were significantly (p < 0.05) higher than birds fed basal diet and the significantly (p < 0.05) least values were obtained in birds fed moringa supplement. In conclusion, three herbal supplements decreased the HSP 70, leptin and adiponectin of broilers exposed to heat stress and moringa supplements lower pro inflammatory cytokine expression in heat stress birds.
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    Lipidomic profile of testicular and epididymal reserves oF West African Dwarf goats
    (Elsevier, 2026) Adeyinka Oye Akintunde; Olwaseyi Emmmanuel Oso; Lois Chidinma Ndubuisi-Ogbonna; Karimat Imam Aliyu; Ayodeji O. Olarinmoye; Olayinka Abosede Ojo
    This study presents a comparative lipidomic analysis of the testicular and epididymal reserves of the West African Dwarf (WAD) goat using gas chromatography–mass spectrometry (GC–MS). Lipid extracts from paired testicular and epididymal tail tissues were analyzed to determine the composition and distribution of fatty acids. Twenty two lipid compounds were identified across all samples, including saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), and sterols. The major fatty acids detected were palmitic acid (C16:0), stearic acid (C18:0), linoleic acid (C18:2), and oleic acid (C18:1). The relative abundance of PUFAs was higher in the epididymal tail compared to the testes, indicating their potential role in sperm membrane fluidity and motility. Cholesterol, an essential membrane component, was relatively consistent across tissues. These findings contribute to understanding lipid metabolism in male reproductive physiology of WAD goats and provide a biochemical basis for fertility enhancement, breed conservation, and improved semen cryopreservation strategies.
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    Metabolic and oxidative status of West African dwarf does at different reproductive stages in southwest Nigeria
    (SpringerOpen, 2019) Abubakar Olatunji Jimoh; Olayinka Abosede Ojo; Uchechi Daureen Gift Ihejirika
    Background: A study was conducted to investigate changes in oxidative stress indicators of West African dwarf (WAD) goats at different reproductive stages. There are a total of 35 dry does, 30 gravid does at first trimester (40– 45 days post-mating), 33 gravid does at second trimester (85–90 days post-mating), 31 gravid does at third trimester (135–140 days post-mating), and 40 postpartum does (30–35 days postpartum). Fastened blood samples were collected from all tagged animals and serum obtained. Serum was assayed for lipid peroxidation, total antioxidant activity, glutathione peroxidase, catalase and superoxide dismutase, serum biochemical, glucose, total protein, albumin, cholesterol, triglyceride, high-density lipoprotein, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, urea, and creatinine using standard procedures. Results: Result revealed that intense metabolic changes were associated with late prepartum and postpartum does as pronounced in oxidative and biochemical indicators of WAD does. Significant differences were found in serum biochemical indices across the different physiological stages of the doe except for triglyceride and low-density lipoprotein. Antioxidant defense is higher at early and mid-gestation and dry does, through low lipid peroxidation and high antioxidant activity as compared to late gestation and lactation does. Conclusion: The observed result shows that the WAD does display oxidative stress during late gestation and lactation owing to intense metabolism. Antioxidant fortification should be employed especially during late gestation and lactation to counteract the oxidative stress in WAD goats.
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    Mitigation effect of aflatoxin on serum biochemical indices of broiler chickens
    (Kwara State University press, 2024) Olayinka Abosede Ojo; Emmanuel Olubisi Ewuola; Immanuel Bitto
    Aflatoxin has been known to be toxic to both crops and livestock. Several methods to mitigate aflatoxin effect have been employed. Knowledge on use of organic methods in aflatoxin mitigation has not been adequately documented. Therefore, effect of aflatoxin bio-control method (Aflasafe) on serum biochemistry of broiler chickens (BC) was investigated. One-day old Marshal BC (n=1020) were allotted to four treatments: Aflasafe maize-based diet (AMBD), farm feed (FF), aflatoxin-contaminated diet with toxin binder (ACDTB) and aflatoxin-contaminated diet without toxin binder (ACDWTB). The experimental design was completely randomised with five replicates (n=255) per treatment for 8 weeks. Blood (5mL) was collected at 8th week and serum was separated and stored for further analysis. Total protein (TP) value of birds fed ACDTWB was significantly lower compared to those of AMBD and FF at the starter and finisher phases. The highest TP values were observed in birds fed AMBD at starter (3.04±0.25g/dl) and finisher (3.41±0.14g/dl) phases. Birds fed AMBD (T1) had highest albumin value (1.39±0.05g/dl).), while birds fed ACDWTB was least (1.26±0.00g/dl).Use of AMBD in poultry is recommended as it has no adverse effect on chickens.
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    Neuro Protective Effect of Lycopene and Tetracarpidium Conophorum Extracts on Heat-Stressed Cockerels
    (Badeggi Journals, 2024-06-01) Olayinka Abosede Ojo
    The study was conducted to determine the neuro protective potential of lycopene and Tetracarpidium conophorum leave Extract (TCLE) on acetyl cholinesterase activity and oxidative status of heat-stress cockerels. A total of 54 cockerels of 25- week old were used for this experiment. There were nine treatments: 0 ml of extract/ 250 ml of water (control)(T1), 30 ml of lycopene/250 ml of water (T2), 60 ml of lycopene/250 ml of water (T3), 30 ml of TCLE/250 ml of water (T4), 60 ml of TCLE /250 ml of water (T5), 15 ml of lycopene + 15 ml of TCLE/250 ml of water (T6), 30 ml of lycopene + 30 ml of TCLE/ 250 ml of water (T7), Vitamin C 0.1g/250 ml of water (T8), Cooler temperature (25±2 o C (T9). Total Protein (TP), brain antioxdant; superoxide peroxidase (SOD), catalase (CAT), Glutathione peroxidase (GPx), Glutathiones-S transferase (GST), Glutathione (GSH), Malondialdehyde (MDA) Acetylcholinesterase (AChe) and phytochemical quantitative/ qualitative analysis of TCLE were evaluated.Results showed that value obtained for crude protein (7.24%), moisture (4.66%), ash and crude fibre content (9.75% and 2.49%) of TCL were depressed, while carbohydrate value (65.30%) was elevated. Also TCLE contain essential compounds as saponin, tannin, phenolics, flavonoids, alkaloids, steroids, glycosides, coumarins, triterpenes, terpenoids and fixed oils, which are present in low quantity(+). There was non-significant effect (P>0.05) of lycopene and Tetracarpidium conophorum on TP, MDA, SOD and catalase of cockerel brain, however, significant elevation was observed in the brain GST was significantly elevated treatment 4 (1490.45 U/mg protein), GPx (1009.04 U/mg protein), GSH (71.03 U/mg protein) and Acetylcholinesterase activities were significantly (P<0.05) influenced by the treatments. It was observed that the experimental inclusions did not significantly impose any negative effect on the cockerels
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    Nutrient Digestibility Coefficients and Carcass Evaluation of Japanese Quails to Aqueous Administration of Egg Lime Molasses Mixture
    (the University of Tehran, 2025) Adeyinka Oye Akintunde; Lois Chidinma Ndubuisi-Ogbonna; Olayinka Abosede Ojo; Bolatito Adenike Shobo; Olufunso Emmanuel Akinboye; Osagie John Afodu; Oluwafunmike Omowunmi Oyekale
    Background:Japanese quails are cheap and easy to maintain, and they have the potential to bridge the gap in animal protein consumption shortage; however, their performance is highly dependent on the efficiency of nutrient utilization. Objectives: This study aimed to determine the effect of an egg-lime-molasses mixture (ELM) administration on Japanese quails’ nutrient utilization and carcass parameters. Methods: The study was conducted at the poultry unit of the Babcock University farmhouse, Ilishan-Remo, Ogun State, Nigeria, from January to March 2022. ELM was prepared by placing fresh chicken eggs in a bowl, after which one liter of lime juice and 500 g of molasses were added and covered tightly for 10 days at 27 °C with a relative humidity of 61%. The solution was then blended. Two-hundred-day-old Japanese quails were assigned to one of five treatments in a completely randomized design. The birds were subdivided into four replicates of 10 birds each. The control (T1) had no administration of ELM; T2, T3, T4, and T5 had inclusion levels of 10, 20, 30, and 40 mL, respectively, all in 500 mL of water. Food and water were provided ad libitum. Data on performance characteristics, apparent digestibility coefficients, and carcass parameters were collected and analyzed using SPSS software, version 22 using descriptive statistics and analysis of variance. Results: The results revealed that ELM did not significantly affect performance characteristics and carcass parameters (P>0.05). The values for digestible crude protein were significantly higher (P<0.05) in the control (62.99%). However, the lowest values were observed among birds administered the lowest dosages (10 and 20 mL ELM with 56.36% and 54.38%, respectively). Birds administered the lowest dosages of ELM (10 mL) had significantly higher (P<0.05) digestible lipids (55.50%) and digestible ash (23.41%) values. The birds administered 20 mL ELM had significantly least (P<0.05) values for digestible lipids (25.50%) and digestible ash (2.58%), while significantly highest (P<0.05) values were observed for the same group of birds for digestible crude fiber (41.05%) and digestible carbohydrates (38.79%). Conclusion: The inclusion of ELM in the water of Japanese quails did not alter their growth performance or carcass parameters.
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    ORAL SUPPLEMENTATION OF CISSUS POLPUNEAAND CITRUS AURANTIFOLIA ENHANCED SERUM BIOCHEMISTRY AND OXIDATIVE STATUS OF TROPICAL RABBITS
    (Association of Organic Agriculture Practitioners of Nigeria (NOAN), 2025) Olayinka Abosede Ojo; Mojeeb K. Abdulazee
    The effect of Cissus populnea stem bark extract (CpSTe), with or without the addition of Citrus aurantifolia extract (CAe), was evaluated on the serum biochemistry and oxidative status of rabbits in the tropics over 34 days. Twenty-four (24) New Zealand White female rabbits were divided into four groups of six rabbits each, designated as Treatment 1-4 (T1-T4). T1 received no extract (Control), T2 was given 2 mL of CAe per kg body weight, T3 received 2 mL of CpSTe per kg body weight, and T4 was given a combination of CAe (1 mL) + CpSTe (1 mL)/kg body weight. At the end of the experiment, a 5 mL blood sample was collected from the marginal ear vein of three rabbits per treatment using a syringe and needle; out of which 3 mLwas dropped into plain bottles to obtain serum for biochemical analysis, while a 2 mL blood sample was dropped into heparinized bottles for hematological assessments The proximate and phytochemical analyses of CPST revealed carbohydrate (67.85%) and tannin (126.60 mg/100 g) as the predominant constituents. Administration of CA significantly (p<0.05) increased the total serum cholesterol in T2, while a decrease was observed in rabbits under T1 (no administration) and T4. Administering 2 mLof CP (T3) significantly (p<0.05) raised the levels of superoxide dismutase (SOD), catalase (CAT), and GSH in the rabbits. The use of CpSTe and CAe separately, rather than combined, could effectively improve the redox status in tropical rabbits.
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    ORAL SUPPLEMENTATION OF CISSUS POLPUNEAAND CITRUS AURANTIFOLIA ENHANCED SERUM BIOCHEMISTRY AND OXIDATIVE STATUS OF TROPICAL RABBITS
    (Association of Organic Agriculture Practitioners in Nigeria (NOAN), 2025) Olayinka Abosede Ojo; Mojeeb K. Abdulazee
    The effect of Cissus populnea stem bark extract (CpSTe), with or without the addition of Citrus aurantifolia extract (CAe), was evaluated on the serum biochemistry and oxidative status of rabbits in the tropics over 34 days. Twenty-four (24) New Zealand White female rabbits were divided into four groups of six rabbits each, designated as Treatment 1-4 (T1-T4). T1 received no extract (Control), T2 was given 2 mL of CAe per kg body weight, T3 received 2 mL of CpSTe per kg body weight, and T4 was given a combination of CAe (1 mL) + CpSTe (1 mL)/kg body weight. At the end of the experiment, a 5 mL blood sample was collected from the marginal ear vein of three rabbits per treatment using a syringe and needle; out of which 3 mLwas dropped into plain bottles to obtain serum for biochemical analysis, while a 2 mL blood sample was dropped into heparinized bottles for hematological assessments The proximate and phytochemical analyses of CPST revealed carbohydrate (67.85%) and tannin (126.60 mg/100 g) as the predominant constituents. Administration of CA significantly (p<0.05) increased the total serum cholesterol in T2, while a decrease was observed in rabbits under T1 (no administration) and T4. Administering 2 mLof CP (T3) significantly (p<0.05) raised the levels of superoxide dismutase (SOD), catalase (CAT), and GSH in the rabbits. The use of CpSTe and CAe separately, rather than combined, could effectively improve the redox status in tropical rabbits.
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    Organ Histopathology of Laying Chickens to Bio Control Methods of Aflatoxin Contamination
    (Sciencedomain International, 2020) Olayinka Abosede Ojo
    Aflatoxin is toxic and carcinogenic to both crops and livestock. Use of different methods of aflatoxin mitigation has not been very effective. Information on the biological methods in aflatoxin mitigation is scanty. Therefore, effect of aflatoxin bio-control method on organ weight and histopathology of layers were investigated. 700 point-of-lay Bovan Nera layers (LC) were randomly allotted to four dietary treatments (Aflasafe maize-based diet AMBD, FF+ toxin binder, Aflatoxin-contaminated diet with toxin binder (ACDTB) and Aflatoxin-contaminated diet without toxin binder (ACDWTB). There were 5 replicates per treatment and experiment lasted for 14 weeks. Histopathology of liver, kidney, spleen, bursa of fabricus and ileum were assessed using standard procedures. Data were analysed using descriptive statistics and ANOVA at α0.05. Bursa histopathology of layers fed ACDTWB showed lymphoid depletion and hepatocellular necrosis, while those fed AMBD showed lymphoid proliferation and hepatocellular aggregates. Layers fed ACDTB and ACDWTB showed severe periportal hepatic degeneration and necrosis, with severe periportal cellular infiltration by mononuclear cells. This was classified as ranging from moderate to severe congestion of the parenchyma as observed in the lungs. The submucosal lymphoid population was expanded in the ileum of layers fed AMBD and those fed FF+toxin binder showed severe villi atrophy. Aflasafe maize-based diet enhanced integrity of the organ weights and histopathology of layers. The use of bio control method of aflatoxin mitigation (aflasafe) in poultry diet is recommended.
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    Organ Weights and Testicular Histology Of Heat-stressed Cockerels Given Lycopene and Tetracarpidium conophorum Leaf Extract
    (Association of Organic Agriculture Practitioners in Nigeria (NOAN), 2021) Olayinka Abosede Ojo
    This study was conducted to assess the relative organ weight and testes histopathology of heat-stressed cockerels given &lycopene and African walnut (Tetracarpidium conophorum Mull. Arg. Hutch & Dalziel.) leaf extract (TCLE). Thirty week (30-week) old cockerels (n=54), were used for this experiment. They were randomly grouped into nine treatments containing: 0 ml of extract / 250 ml of water (control) (T1), 7.5 ml of lycopene / 250 ml of water (T2), 15 ml of lycopene / 250 ml of water (T3), 7.5 ml of TCLE / 250 ml of water (T4), 15 ml of TCLE / 250 ml of water (T5), 7.5 ml of lycopene + 7.5 ml of TCLE / 250 ml of water (T6), 15 ml of lycopene + 15 ml of TCLE / 250 ml of water (T7), Vitamin C 0.1 g per 250 ml of water (T8), Cold temperature + Cold water (5-7OC) (T9). The result showed that there was non-signicant effect (P>0.05) of lycopene and TCLE on all the relative organs weight values recorded. Testes of the cockerels given the control treatment (T8) had abnormal widening of interstitial spaces and degeneration of interstitial cells and lumen. For cockerels on T2 diets, there was an increase in both the interstitial spaces and intracellular spaces of the seminiferous tubules. Cockerels given T7 showed appreciable normal histomorphology with increased interstitial spaces and intracellular spaces of the seminiferous tubules. Cockerels under T8, given Vitamin C, showed abnormal widening of interstitial spaces and degeneration of interstitial cells of the seminiferous tubules. Normal appearance without abnormal widening of interstitial spaces and degeneration of interstitial cells in cockerel given T9. In conclusion, 15 ml of lycopene + 15 ml of TCLE / 250 ml of water (T7) can be administered to cockerels in a cool environment, with an addition of cold water (T9), to give a better testicular structure, and 15 ml of lycopene / 250 ml for improved internal organ weight.
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    Performance, hemato-biochemical indices and oxidative stress markers of broiler chicken fed phytogenic during heat stress condition
    (Korean Society of Animal Science and Technology, 2022) Olatunji Abubakar Jimoh; Olajumoke Temidayo Daramola; Hafsat Ololade Okin-Aminu; Olayinka Abosede Ojo
    Thermal stress is a tremendous health predicament encountered by poultry farmers with adverse effects on the performance, product stature, health condition, survival, and overall welfare of poultry birds, and so requires urgent dietary user-friendly strategy to curb. This study was conducted with 200-day old broilers for the purpose of investigating the potential of phytogenics in refining the negative effects of heat stress on broiler chicken. Moringa, Phyllanthus and mistletoe leaves were processed as phytogenic supplements and incorpo rated into standard ration for broilers as treatments B1 (control), B2, B3 and B4 diet during the peak of thermal discomfort in humid tropics. Growth and carcass indices were monitored in a 49-day trial and blood samples were harvested at the end of the ordeal period to assess haematology, serum biochemical and oxidative stress markers with the use of standard pro cedures. The results obtained showed that the prevailing environmental condition in the study site indicated that the birds were exposed to heat stress. Birds fed on moringa and mistletoe supplements had higher performance index than birds without supplementation during heat stress condition, while birds fed on mistletoe supplement had the highest survival rate across the treatments. The liveweight, slaughter weight, dressed weight and eviscerated weight of heat stressed birds fed on moringa, phyllanthus and mistletoe supplements were significantly higher than birds on control treatment. Heterophyl/lymphocyte ratio of heat stressed birds without supplement were higher than birds on phytogenic supplements, with least values recorded in phyllanthus and mistletoe fed birds. Birds on phytogenic supplement tend to have lower cholesterol profile, lipid peroxidation and better antioxidant profile than birds on control treatment during heat stress conditions. Mistletoe supplementation in broiler ration enhances the survival rate, as well as promotes growth indices better among the phytogenic supplements. However, phytogenic supplements did ameliorate the negative effects of thermal discomfort on performance, physiological and oxidative stress in heat-stressed broiler chicken.
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    Reproductive Potentials of Female Japanese Quails to Administration of Egg-Lime-Molasses Mixture
    (2985-1211, 2023) Adeyinka Oye Akintunde; Lois Chidinma Ndubuisi-Ogbonna; Mofiyinfoluwa Modupe Ladele; Oladapo Ayodeji Olorunfemi; Olayinka Abosede Ojo; Olufunso Emmanuel Akinboye; Bolatito Adenike Shobo; Osagie John Afodu
    Japanese quails are hardy with huge potentials to bridge protein deficiency, hence the need for an assessment of their reproductive potentials. The egg-lime-molasses mixture (ELM) was prepared by placing 10 fresh chicken eggs (average weight of 58 g per egg) in a bowl, after which 1 liter of lime juice and 500 g of molasses were added, then it was covered and left for 10 days. The solution was then blended. Two hundred day-old Japanese quails were assigned to 5 treatments with 40 birds in a completely randomized design (CRD) and 4 replicates of 10 birds each. The control (T1) had no ELM, while treatments 2, 3, 4 and 5 (T2, T3, T4, and T5) had inclusions of 2%, 4%, 6%, and 8% ELM in water, respectively. Data were collected on the morphometrics, egg characteristics, follicle stimulating, and leutenizing hormone concentrations. The findings revealed that at the early stage of laying, the administration of ELM did not have a significant effect (p > 0.05) on egg parameters. However, at the latter phase of egg laying, there was a significant effect (p < 0.05) on shell weight and albumen percentage. ELM did not have a significant effect (p > 0.05) on the production performance. There was no significant effect (p < 0.05) on follicular characteristics, while birds administered 30ml ELM/500ml of water had the significantly highest (p < 0.05) weights of oviduct (1.01 ± 0.01 g) and ovary (3.995 ± 0.005 g). Birds administered higher levels of ELM had significantly higher concentrations (p < 0.05) of leutenizing and follicle stimulating hormones. The inclusion of aqueous administration of ELM in drinking water has no detrimental effect on the reproductive potentials of female Japanese quails.
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