Browsing by Author "M.E. Balogun"

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    Effect of Acute Administration of Ethanol Extract of Cannabis sativa Leaf on Oxidative Stress Biomarkers in Male and Female Wistar Rats
    (Society for Experimental Biology of Nigeria (NISEB), 2023-06-30) Amuda Oluwasola; M.E. Balogun; A.F. Odetayo; O.E. Ayoola; S.S. Muzzammill; E.B. Mariam; S.O. Zainab; V.J. Nkemdili; O.J. Adekunle; O.J. Deborah; N.H. Aishat; A.M. Comfort
    Oxidative stress is a phenomenon caused by an imbalance between production and accumulation of oxygen reactive species (ROS) in the cells and tissues and the ability of a biological system to detoxify these reactive products. This study investigated the acute effect of different doses of ethanolic extract annabis sativa (EECS) on oxidative stress biomarkers in male (M) and female (F) Wister rats. Twenty male rats (170 g ± 1.24) and twenty female rats (150 g ± 1.05) were separately assigned into four groups of five animals each for male and female, such that the rats in groups IM and IF, IIM and IIF, IIIM and IIIF and IVM and IVF received orally 1 mL of distilled water, 2 mg/kg body weight (BW) of EECS, 4 mg/kg BW of EECS and 6 mg/kg BW of EECS respectively for twenty one (21) days. Catalase, superoxide dismutase (SOD), Glutathione peroxidase (GPx), Glutathione reductase (GSH), malondialdehyde (MDA), total antioxidant capacity (TAC) and lactate dehydrogenase (LDH) were determined using standard methods. Administration of different doses (2 mg/kg BW, 4 mg/kg BW and 6 mg/kg BW) of EECS significantly (p<0.05) decreased catalase, SOD, GPx, GSH and TAC levels respectively when compared with the control. However, Cannabis sativa (CS) increased MDA and LDH levels significantly (p<0.05) when compared with the control. It was deduced that these alterations in oxidative stress biomarkers were dependent on the doses of CS consumed. Thus, the more the concentration of CS consumed, the more it may affect oxidative stress biomarkers negatively. In addition, all these effects of CS on oxidative stress biomarkers were more in male than in female.
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    The Ameliorative effects of melatonin on cannabis-induced liver toxicity in male and female wistar rats.
    (African Journals Online, 2023-08-23) A. Oluwasola; O.E. Ayoola; Garba Saa'du; M.A. Adepoju; M.E. Balogun; S.A. Biliaminu; L.A. OLayaki
    Liver has an incontrovertible influence on several functions of many organs in the body. It is prone to xenobiotic induced injury due to its central role in xenobiotic metabolism and its portal location within the system. This study investigated and compared the effects of melatonin on cannabis-induced liver toxicity in male and female wistar rats. Twenty female rats (156g ± 1.05) and twenty male rats (192g ± 1.42) were separately assigned into four groups of five animals each, such that the rats in groups I, II, III and IV received orally 1mL of distilled water, 2mg/kg body weight (BW) of ethanolic extract of Cannabis sativa EECS, 2mg/kg BW of EECS plus 4mg/kg BW ofmelatonin and 4mg/kg BW of melatonin respectively for twenty one days. Creatinine, bilirubin, gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP),lactate dehydrogenase (LDH), alanine amino transferase (ALT), aspartate amino transferase (AST), albumin andinducible protein were determined using standard methods. Cannabis sativa(CS)significantly(p<0.05) increased creatinine, bilirubin, GGT, ALP, LDH, ALT, and AST respectively. CS also decreased albumin and inducible protein significantly (p<0.05). However, these increments and decrements were more in male than female rats. All these effects were ameliorated to the level comparable with the control when the CS was administered with melatonin CS showed alterations in liver biomarkers (enzymes) which were probably associated with liver toxicity. These alterations were more in male than in female. However, these effects were ameliorated by melatonin. Since the consumption of CS is increasing globally because of its medical uses, thus, consumption of melatonin as supplement may be recommended.