Browsing by Author "L.A. Olayaki"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Ameliorative Effect of Melatonin on Reproductive Hormones in Ethanol Extracts of Cannabis sativa-Treated Female Wistar Rats
    (The Nigerian Society for Experimental Biology (NISEB), 2022) A. Oluwasola; O.E. Ayoola; A. F. Odetayo; Garba Saa’du; L.A. Olayaki
    This study investigated the effects of melatonin and ethanol extracts of Cannabis-sativa on reproductive hormones in female rats. Twenty female rats were assigned into four groups of five animals each, such that the rats in groups 1, 2, 3and 4 received orally 1mL distilled water, 2mg/kg of ethanol extract of Cannabis sativa (EECS), 2mg/kg of EECS+4mg/kg of melatonin and 4mg/kg of melatonin, respectively for 14 days. Gonadotropin releasing hormone (GnRH), follicle stimulating hormone (FSH), luteinizing hormone (LH), oestradiol (E), progesterone and prolactin were quantified according to the instruction provided by assay kit manufacturers, using microplate immunoenzymometric (EMA/ELISA) assays. EECS significantly (p<0.05) decreased GnRH, FSH, LH, E, progesterone and prolactin levels respectively when compared with the control. However, co-administration of melatonin with EECS significantly (p<0.05) increased all the aforementioned reproductive hormones to the level comparable with the control. Histological study of hypothalamus and pituitary gland revealed that EECS-treated rats showed scanty and shrink nissl bodies and severe degeneration of acidophils and basophils when compared with the control. These pathological changes were ameliorated by melatonin. This study concluded that the effects of CS on reproductive hormones could be mediated by damage to the endocrine system. However, these effects of EECS on the reproductive hormones could be ameliorated by melatonin. The study recommended that consumption of melatonin could be used as supplement to prevent the side effects of EECS on the reproductive hormones.