Browsing by Author "Issa, B.T."

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    Assay of Bioactive Compounds in Stored Kolanuts
    (Journal of the faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria, 2024-03) Olahan, G.S.; Ajadi, I.; Issa, B.T.
    Kolanuts are widely consumed by humans. The medicinal and therapeutic potentials of plants are credited to their bioactive compounds. This study was aimed at investigating the bioactive compounds in kolanuts from Ifelodun and Odo-Otin Local Government Areas of Osun State, popular kolanut producing LGAs in Nigeria. The bioactive compounds were identified using Gas Chromatography-Mass Spectrometry (CG-MS) to analyse crude extracts of the kolanuts using acetone:methanol (1:1 v/v) as the extractant. Twenty five(25) bioactive compounds were identified, with 9-Octadecenoic acid, methyl ester (32.91%) being the predominant compound. Six (6) of the bioactive compounds were common in all the kolanuts (caffeine, hexadecanoic acid, methyl ester, n-hexadecanoic acid, 9, 12-Octadecadienoic acid, methyl ester (z,z), methyl stearate, and phthalic acid, di(oct-3-yl) ester). Some of the bioactive compounds were identified only in the kolanuts from Ifelodun L.G.A. e.g. nonanoic acid, methyl-2-octylcyclopropene-1-heptanoate, 9octadecanoic acid (z), 9-octadecanoic acid. Also, 11-octadecanoic acid, methyl ester (z), 2chloroethyl linoleate, bicyclo (4.1.0) heptane, etc were present only in the kolants from Odo-Otin L.G.A. Out of the 25 bioactive compounds identified, only 12 have been reported to be present in kolanuts some of e.g. caffeine, n-Hexadecanoic acid, etc, while the remaining 13 have been reported in other plants other than kolanuts
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    Identification of Fungi Associated with Rotten Sweet Orange Fruits (Citrus sinensis L.) in University of Ilorin Botanical Garden
    (Federal University Birnin-Kebbi, 2023-12) Olahan, G.S.; Ajadi, I.; Issa, B.T.
    The presence of fungi in oranges poses a significant risk to public health, impacting global economies and the well-being of both humans and animals. To effectively address this problem, it is important to identify the specific types of fungi to address this concern effectively. This study was aimed at isolating and characterizing fungal species associated with rotten sweet oranges (Citrus sinensis L.) in the University of Ilorin using phenotypic characterization and molecular tools. A total of five orange fruits were collected from different trees at the Unilorin botanical garden and immediately transported to the Biology laboratory, University of Ilorin. The rotten part of the fruits was cut out, surface-sterilized, and then inoculated onto potato dextrose agar. Aspergillus flavus, Aspergillus niger and Rhizopus arrhizus were the fungal isolated and identified. The identification of these pathogens is crucial for implementing effective post-harvest management measures. This will aid in eliminating the pathogens from sweet orange fruits during post-harvest handling, reduces spoilage and extending their shelf life.