Browsing by Author "Ben-Uwabor, P.O."

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    Characterization of Fungal Contaminants in Fermented Locust Beans in Kwara State, Nigeria: Implications for Food Safety
    (Federal University of Lafia, Nasarawa State, Nigeria, 2025-12) Olahan, G.S.; Ajadi, I.; Ben-Uwabor, P.O.; Adebayo, S.E.
    Fermentedlocust bean seeds are utilized for seasoning in various traditional dishes. Its production is mostly under unhygienic conditions by majorly illiterate women who care less about microbial contamination of the final product. Consumption of any contaminated food can cause health complications in humans. This study was aimed at isolating the mycoflora associated with fermented locust bean seeds collected from three villages in Kwara State, Nigeria using both morphological and molecular techniques. DNA of each of the isolates was extracted using Zymo Fungal/Bacteria DNA Miniprep Kit. PCR amplification of the ITS regions of the isolates was carried out using primer pair; ITS1 and ITS4. The products had been sequenced, and the results from the BLAST search revealedthat Isolate A was Aspergillus flavus, Isolate B was A. niger, Isolate C was Rhizopus arrhizus, and Isolate D was Mucor indicus.The fermented locust bean seeds from Madi village had the highest fungal count (7.2 X 103cfu/gm), while that from Ogundele village had the lowest (1.4 X 103cfu/gm). The presence of Aspergillus nigerand A. flavus (mycotoxigenic fungi) in this study poses a significant public health risk. Therefore, it is important to encourage the villagers involved in fermented locust bean production and storage in these villages to be more hygienic.
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    FUNGICIDAL EFFECT OF TEAK (TECTONA GRANDIS L.) LEAF EXTRACTS ON FUSARIUM OXYSPORUM
    (West University of Timisoara, series of Biology., 2023-12) Olahan, G.S.; Ajadi, I.; Ben-Uwabor, P.O.
    Fusarium oxysporum f. sp. lycopersici is the pathogen of tomato wilt, a disease of great economic importance worldwide. Although application of synthetic fungicides such as thiophanate methyl and mancozeb can prevent the occurrence of this disease, their effects on the physical environment especially, is a limiting factor. Botanicals, i.e. plantbased fungicides are now being preffered for controlling fungal pathogens because they have minimal environmental impact and are less dangerous to consumers in contrast to synthetic fungicides. Teak leaves have been reported to possess a very great antimicrobial activity because of their high content of phytochemicals. In an effort to develop eco-friendly chemical strategy for control of Fusarium wilt disease of tomato plants, in vitro effect of 10, 30 and 50% (w/v) concentrations of the aqueous and ethanolic leaf extracts of teak (Tectona grandis L.) on the radial growth of the mycelia of Fusarium oxysporum f. sp. lycopersici was investigated using the pour plate method. Results of the study revealed that both extracts retarded the radial growth of mycelia of F. oxysporum f. sp. lycopersici compared to that of the control, with the ethanolic extract having a greater effect at the concentrations tested in this study. It is therefore recommended that an in vivo study of effects of the same leaf extracts on wilt – infected tomato plants be conducted