Browsing by Author "Babatunde, S.K."

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    Isolation and Identification of microorganisms in comb and body parts of wild and domesticated honey bees of two ecozones of Nigeria
    (Erudite Journal of Microbiology and Biodiversity, 2013) Ajao, Adeyemi Mufutau; Babatunde, S.K.
    The survival of the honey bee depends on the successful defense against microbial invaders, parasites and predators. This research was carried out to isolate and identify microorganisms associated with the honeybees of two ecological zones of Nigeria. Thirty adult worker honeybees were collected from the wild, modern and traditional beekeeping methods and analyzed for microbial presence. The body and bee comb were swabbed using sterile normal saline moistened swab stick. The swabs were diluted, homogenized and cultured for microbial growth. The contents of the fore, mid and hind guts were emptied, homogenized and cultured. 1ml of homogenized sample was diluted, from this into four sterile dilutions of 1/20 1/40 1/80 and 1/160. 1.0 ml each of dilutions which was inoculated into molten Sabourand dextrose Agar (SDA) containing tetracycline for fungi growth, while nutrient agar and De Ma Rogossa Sharbroth (MRS) agar were inoculated for the bacteria. SDA plates were incubated at 30o C for 3 days while SDA and MRS were incubated at 37oC overnight. The total number of colonies forming units (CFU) per plate was counted and was correlated to the dilution factor. Each different colony was identified using morphological and biochemical tests. The microbial examination of the comb, body and gut of the bees showed the presence of six moulds and fungi: Mucor hiemalis, Penincillium frequentans, Aspergilus repens, Rhizopus stolonifer, Geotrichums sp. and Botryotricum sp. and three bacteria species in the three beekeeping methods in both ecological zones. The species of the bacteria encountered were: Staphylococcus aureus, Streptobaccillus sp. and Baccillus pulyifacien. Some of the isolated microbes such as Mucor hiemalis, Aspergilus species and Rhizopus species have been implicated as causative organisms of chalkbrood disease of bee. Further research is been suggested for the roles of the isolates in much dreaded CCD.
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    Itaconic acid production by Aspergillus terreus using pod of Moringa oleifera as substrate in solid state fermentation.
    (Nigerian Society of Microbiology, 2018) Ajiboye, A.E.; Babatunde, S.K.; Adedayo, M.R.; Ajuwon, I.B.; labinjo, Z.Y
    Itaconic acid was produced by Aspergillus terreus under solid state fermentation using the pod of Moringa oleifera as substrate. Grinded Moringa pod was weighed into seperate shake flasks containing salts and Aspergillus terreus (3.2 x 104 spores/ml ) was added. The substrate was left to ferment for 5 days. Carboxylmethylcellulose (CMC) was used as control substrate. Itaconic acid was assayed using standard procedures. Different fermentation parameters such as substrate concentration, inoculum size and incubation period were varied to determine the maximum production of itaconic acid. Light spectrophotometer was used to measure the absorbance of the itaconic acid concentration produced after each day of fermentation at 385nm. The maximum yield of itaconic acid of 18.39mg/ml was obtained with a substrate concentration of 10g while 17.2mg/ml was produced with an inoculum size of 6ml (3.2 x 104 cfu/ml ) and 13.91mg/ml on day 5. It is concluded that the pod of Moringa oleifera can be used as a substrate for itaconic acid production using A. terreus in solid state fermentation.
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    Mycoflora of Phoenix Dactylifera (Date Palm) Fruit Sold in Some Markets in Ilorin
    (Globe edu Group www.theijst.com, 2016) Adedayo, M.R.; Babatunde, S.K.; Ajiboye, A.E.; Adetuyi, E.O.
    Aim: the research was conducted to isolate and characterize spoilage mycoflora present on dried date palm fruit commonly sold in some markets within Ilorin metropolis. Method: samples were collected from eight different market locations within Ilorin metropolis. The samples were surface sterilized and split opened to isolate fungi from them and the isolates were characterized morphologically on plate and under the microscope. Characteristics observed were compared with literature for the identification of the specimen following standard methods. Physicochemical analysis was also conducted on the samples according to standard techniques. Result: A total number of six fungi were isolated and identified as Apergillus niger, Neurospora crasa, Aspergillus fumigatus, Aspergillusflavus, Penicillum chrysogenum, and Syncaehalastron racemosus withA. niger having the highest percentage of occurrence. The physicochemical analysis revealed that the samples has high percentage of sugar and mineral contents. Conclusion:the study revealed that date palm fruit sold within Ilorin metropolis is heavily contaminated with fungi. The fungi responsible for its spoilage are those that have been known to produce toxic substances which have adverse effect on the consumer’s health.
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    Prevalence and antibiotic resistance of Escherichia coli O157:H7 in beef at a commercial slaughterhouse in Moro, Kwara State, Nigeria
    (Microbiology Society, 2021) Ajuwon. B.I.; Babatunde, S.K.; Kolawole, O.M.; Ajiboye, A.E.; Lawal, A.H
    Background. Gastroenteritis due to foodborne disease is a leading cause of death in developing countries. In Nigeria, there is an increasing demand for beef. Yet, there is no surveillance for Escherichia coli O157:H7 contamination of raw beef and little is known about the carriage of this pathogen in Nigeria’s livestock. Methods. A total of 415 samples, including 180 cow carcass swabs, 180 caecal content samples, 16 water samples, 25 hand swabs and 14 knife swabs were collected at a large abattoir in the Moro region of Kwara State, Nigeria. The samples were enriched in modified tryptone broth containing novobiocine, and plated onto Sorbitol–MacConkey agar (Oxoid SR0172E) supple- mented with 0.05 mg l−1 cefixime and 2.5 mg l−1 potassium tellurite (Oxoid) (CT-SMAC). Indole- producing isolates were confirmed serologically by serotyping with antisera specific for the O157 and H7 antigens. The E. coli O157:H7 isolates were further tested for their susceptibility to antibiotic agents using the disc diffusion method. Commercially available Gram-negative multi- discs (Oxoid) comprising nitrofurantoin (30 μg), ampicillin (5 μg), ceftazidime (30 μg), gentamicin (10 μg), ciprofloxacin (5 μg), augmen- tin (30 μg), ofloxacin (5 μg) and cefuroxime (30 μg) were tested. Results. Overall, 16 (3.9 %) samples were contaminated with E. coli O157:H7, of which 10 (5.6 %) were isolated from carcass swabs, 4 (2.2 %) from caecal content samples and 2 (12.5 %) from water. All isolates were multidrug-resistant (MDR), with resist- ance to ampicillin, ceftazidime and cefuroxime being the most common. Conclusion. This study provides evidence to suggest that E. coli O157:H7 exists in the beef production chain. The pathogen reveals a high frequency of multidrug resistance, suggesting that consumers and handlers of such meat are at risk of contract- ing antibiotic- resistant E. coli O157:H7-associated foodborne disease. Routine monitoring of antibiotic resistance is critical to uncovering novel therapeutic strategies that will help inform clinical practice guidelines.