Browsing by Author "Ajao, A. T."

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    Biosurfactant Production and Characterization by Bacillus altitudinis Strain LCDB-BP3 Isolated from Pesticide-Contaminated Soil in Kwara State, Nigeria
    (Jewel Journal of Scientific Research (JJSR). Federal University of Kashere-Nigeria, 2024) Abdulsalam Z. B.; Ajao, A. T.; Aborisade, W. T.; Jimoh, F. A.
    Microbial biosurfactants have gained attention as promising alternatives for bioremediation, particularly for pesticide-contaminated soils, due to their biodegradability and non-toxic nature. These biosurfactants also hold potential for various industrial applications, including food, petroleum, and fertilizer industries, and may serve therapeutic roles as adjuvants. However, there remains a need to produce biosurfactants using indigenous bacterial isolates to address environmental pollution sustainably. In this study, biosurfactant was produced using Bacillus altitudinis strain LCDB-BP3, identified as a Lambda Cyhalothrin-Degrading Bacterium (LCDB- BP3) in a previous research. The biosurfactant-producing ability of the isolate was confirmed by oil displacement (14.32 cm), emulsification activity (70%), and beta-hemolytic activity on blood agar. Stability tests revealed the biosurfactant's resilience across a wide range of temperatures (up to 105°C), pH (3–13), and salinity (3–7%), making it suitable for diverse environmental conditions. Thin-layer chromatography (TLC) and Fourier-transform infrared (FTIR) spectroscopy further confirmed the presence of functional groups such as O-H, N-H, ester carbonyl, and C-H, supporting the lipopeptide nature of the biosurfactant. These findings highlight the potential of the biosurfactant produced by Bacillus altitudinis strain LCDB-BP3 for use in the bioremediation of pesticide-contaminated soils and offer a greener alternative to chemical agents in industrial settings.
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    Isolation and Screening for Potential endosulfan-degrading bacteria from Soil
    (Dutse Journal of Pure and Applied Sciences, 2024) Aborisade, W. T.; Ajao, A. T.; Ntagbu, F. G.; Bale, S. I
    The urgent need for sustainable management of persistent organic pollutants, due to their negative impact on the environment, is of great concern to environmental stakeholders. In this study, the potential endosulfan-degrading (PED) bacteria were screened and identified from fresh soil samples collected from the Teaching and Research farm of Kwara State University, Nigeria. The bacterial strains were isolated using enrichment techniques and characterized through morphological, biochemical, and molecular analyses. Their potential for endosulfan utilization was assessed in an endosulfan supplemented mineral salt medium. Three potential endosulfan-degrading (PED) bacterial strains isolated were Achromobacter xylosoxidans strain PED1, Pseudomonas alkylphenolica strain PED2, and Sphingomonas sp. Strain PED3 with the accession numbers MF973060, MF973061 and MF973062 respectively. The optical density (OD) values increased for all the isolates by the third day, and the highest OD values of 0.51 (PED1), 0.52 (PED2) and 0.59 (PED3) were recorded on the ninth day. The observed increase in population density of the isolates in the endosulfan-supplemented mineral salt medium suggests their capability to utilize the endosulfan compound. Further study is required to evaluate the suitability of these strains for bioremediation purposes.
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    Water quality assessment and bacteriological evaluation of fishpond in Ilorin
    (Aswan University Journal of Environmental Studies (AUJES), 2023) Idemudia, I. B.; Aborisade, W. T.; Ajao, A. T.
    Good water quality is essential for a productive aquaculture system and to safeguard the health of the consumer. We assessed the quality of fishpond water in Phase 1, Mubo-Royal Valley fish farm in Ilorin, Kwara State, Nigeria. Water samples were collected in the 1st and 3rd weeks of fish breeding from three earthen and two concrete fishponds. The bacteriological and physicochemical parameters of the samples were determined in line with standard methods. Results showed that the pH, temperature, and dissolved oxygen (DO) values were within the recommended range of the National Environmental Standard and Regulations Enforcement Agency (NESREA). However, higher chemical oxygen demand (COD) values above the NESREA recommended range were recorded in the 1st and 3rd weeks of sampling in earthen Pond 1 and Pond 2, respectively. The Nitrate (NO3 2- ) and phosphate (PO4 2- ) contents of all sampling ponds were within the acceptable values of NESREA in the 1st week of sampling. While significant (p < 0.05) increases in NO3 2- and PO4 2- were observed in earthen ponds in the 3rd week. Copper and iron (mg/l) values were higher than NESREA recommended range, while lead and chromium values were within the range. Bacterial loads varied with the period of sampling with significant (p < 0.05) increases in the population of heterotrophic bacteria and Salmonella Shigella in the 3rd week of sampling. The nine bacterial genera isolated were Pseudomonas sp., Micrococcus sp., Staphylococcus sp., Salmonella sp., Escherichia coli, Enterobacter sp., Proteus sp., Streptococcus sp. and Bacillus sp. All the isolates were present in earthen and concrete ponds except Proteus sp. and Streptococcus sp. were absent in concrete ponds. Pseudomonas sp. had the highest frequency of occurrence while Streptococcus sp. had the lowest. Regular monitoring of water quality parameters is recommended to prevent potential risks associated with potential pathogens in fishponds